八味中药对绵羊卵巢颗粒细胞增殖与保护的影响

发布时间：2018-05-31 23:43

本文选题：中药 + 颗粒细胞　；参考：《甘肃农业大学》2015年硕士论文

【摘要】：本研究利用细胞培养技术,观察了八味中药对绵羊卵巢颗粒细胞离体培养的影响;运用分子生物学、荧光定量等方法检测氯丙嗪、H2O2染毒对绵羊卵巢颗粒细胞凋亡基因Bax、Bcl-2 mRNA表达的影响;研究了八味补虚中药对绵羊卵巢颗粒细胞增殖效果以及对染毒后的颗粒细胞的保护作用。研究结果如下:1、体外培养颗粒细胞,密度小于103个/ml时,颗粒细胞会生长缓慢,会出现变形现象。体外适宜培养密度为106个/ml左右。2、颗粒细胞培养过程中,黄芪、刺五加、龙眼肉、枸杞、女贞子、蛤蚧、菟丝子对颗粒细胞增殖具有促进作用,蛤蚧(1g/L、1×10-3g/L)、菟丝子(1×10-2 g/L、1×10-3g/L)、女贞子(1g/L、1×10-1g/L、1×10-2g/L、1×10-3g/L)、枸杞子(1×10-2g/L、1×10-3g/L)、黄芪(1×10-3g/L)对卵巢颗粒细胞增殖极显著(P0.01),而熟地没有显著促进作用。3、颗粒细胞染毒试验中,氯丙嗪浓度在0.1μmol/L以上对颗粒细胞生长抑制作用极显著(P0.01),H2O2浓度在150μmol/L以上对颗粒细胞增殖抑制极显著(P0.01)。随着氯丙嗪、H2O2染毒浓度的增加,Bax、Bcl-2基因mRNA表达上调。4、黄芪、刺五加、熟地、龙眼肉、枸杞、女贞子、蛤蚧、菟丝子对氯丙嗪诱导的颗粒细胞损伤具有抑制作用。黄芪、刺五加、熟地、龙眼肉、枸杞子、女贞子、蛤蚧、菟丝子对H2O2引起颗粒细胞损伤具有抑制作用。
[Abstract]:In this study, the effects of eight herbs on the in vitro culture of ovaries granulosa cells in sheep were observed by cell culture technique, and molecular biology was used. The effects of chlorpromazine and H _ 2O _ 2 on the expression of Bcl-2 mRNA in ovaries granulosa cells were detected by fluorescence quantitative method, and the protective effects of BWBX on the proliferation of ovaries granulosa cells and the protective effects on granulosa cells after exposure to chlorpromazine were studied. The results are as follows: 1: 1. When the density of granulosa cells is less than 103 / ml, granulosa cells will grow slowly and deform. The suitable culture density in vitro was about 106 / ml. In the process of granulosa cell culture, Astragalus membranaceus, Acanthopanax senticosus, longan flesh, Lycium barbarum, Ligustrum lucidum, Gekko gecko and Cuscuta chinensis could promote the proliferation of granulosa cells. 1 g / L 1 脳 10 -3 g / L, 1 脳 10 -2 g / L 1 脳 10 -3 g / L, 1 g / L Li 1 脳 10 -1 g / L 1 脳 10 -2 g / L 1 脳 10 -3 g / L, 1 脳 10 -2 g / L 1 脳 10 -3 g / L Li, 1 脳 10 -2 g / L 1 脳 10 -3 g / L, 1 脳 10 -3 g / L). The inhibitory effect of chlorpromazine above 0.1 渭 mol/L on the growth of granulosa cells was extremely significant. The inhibitory effect of H2O2 concentration above 150 渭 mol/L on the proliferation of granulosa cells was extremely significant. With the increase of the concentration of chlorpromazine and H _ 2O _ 2, the expression of Bcl-2 gene mRNA was up-regulated. Astragalus membranaceus, Acanthopanax senticosus, ripe ground, longan, Lycium barbarum, Ligustrum lucidum, Gecko and Cuscuta chinensis could inhibit the damage of granulosa cells induced by chlorpromazine. Astragalus, Acanthopanax senticosus, ripe ground, longan, wolfberry, Ligustrum lucidum, Gekko, Cuscuta have inhibitory effect on H2O2 induced granulosa cell damage.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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