大庆市及周边地区非免疫猪群猪圆环病毒感染情况调查

发布时间：2018-06-01 09:17

本文选题：猪圆环病毒 + 酶联免疫吸附试验　；参考：《动物医学进展》2017年01期

【摘要】：为了解大庆市及周边地区猪群中PCV的感染情况,应用ELISA和PCR技术对采自大庆市5区4县及周边地区55个村屯/猪场未免疫PCV2疫苗猪血清样本649份,5家病猪场和6家屠宰场组织样本448套,进行血清学和分子生物学检查,并对检测结果进行了比较分析。结果显示,大庆市及周边地区未免疫PCV2疫苗猪血清样品PCV2抗体平均阳性率为54.70%,其中断奶仔猪样品阳性率为50.68%,育肥猪样品为70.00%,繁育母猪样品为54.70%。组织样品PCV1阳性率为4.46%,PCV2阳性率为22.32%。调查结果可为确定地区流行毒株序列提供原始材料和制定科学防控措施提供依据。
[Abstract]:In order to understand the infection of PCV in pigs in Daqing city and its surrounding areas, ELISA and PCR techniques were used to detect the serological and molecular biology of 649 samples of unimmunized PCV2 vaccine serum from 5 districts, 4 counties and 5 villages and surrounding areas of Daqing, including 6 slaughterhouses and 5 diseased pig farms. The test results were compared and analyzed. The results showed that the average positive rate of PCV2 antibody in serum samples of unimmunized PCV2 vaccine pigs in Daqing and its surrounding areas was 54.70, among which the positive rate of weaning piglets was 50.68, that of fattening pigs was 70.00,and that of breeding sows was 54.70. The positive rate of PCV1 in tissue sample was 4.46 and the positive rate of PCV2 was 22.32. The results of investigation can provide the basis for determining the sequence of epidemic strains in the region and making scientific prevention and control measures.
【作者单位】：大庆市动物疫病预防与控制中心;
【基金】：2014年黑龙江省级领军人才梯队后备带头人资助项目
【分类号】：S858.28

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