肌注氟苯尼考在鸭疫里氏杆菌感染鸭体内的药动学特征

发布时间：2018-06-01 15:46

  本文选题：氟苯尼考 + 鸭疫里氏杆菌　； 参考：《中国农业科学》2017年20期

【摘要】：【目的】研究并比较肌注氟苯尼考在2周龄健康和鸭疫里氏杆菌感染鸭体内的药代动力学特征。【方法】240只鸭随机分为2组,各120只鸭,其中一组用鸭疫里氏杆菌腿部感染,分别以30 mg·kg~(-1)体重单剂量肌内注射氟苯尼考,采血点为给药前和给药后5、10、15、20、30、45 min和1、1.5、2、4、8、10、14 h。采用高效液相色谱法(HPLC)测定血浆氟苯尼考的浓度,采用紫外检测器检测,检测波长为223 nm,流动相为乙腈和水,其体积比为26和74,流速为1 m L·min-1,色谱柱为Agilent C18(4.6 mm×150 mm,5μm),进样量为20μL。药动学分析软件Win Nonlin 5.2.1以非房室模型拟合处理血药浓度-时间数据,计算相关的药动学参数,并用统计学软件SPSS17.0对药动学参数进行t检验统计学分析。【结果】氟苯尼考在健康鸭体内的峰浓度(Cmax)为(22.88±3.11)μg·m L-1,表观分布容积(Vd/F)为(2.39±0.81)L·kg~(-1),体清除率(ClB/F)为(0.64±0.11)L·h-1·kg~(-1),消除半衰期(t1/2β)为(2.57±0.51)h和药时曲线下面积(AUC)为(47.28±7.87)μg·m L-1·h;氟苯尼考在感染鸭体内的峰浓度(Cmax)为(19.77±1.82)μg·m L-1,表观分布容积(Vd/F)为(2.44±0.46)L·kg~(-1),体清除率(ClB/F)为(0.63±0.08)L·h-1·kg~(-1),消除半衰期(t1/2β)为(2.74±0.54)h和药时曲线下面积(AUC)为(48.11±6.62)μg·m L-1·h。统计分析氟苯尼考在健康鸭和感染鸭的药动学参数,结果表明,除了Cmax差异显著(P0.05),其他参数差异均不显著(P0.05)。【结论】两者的主要药动学参数相比较,感染鸭体内的Cmax显著低于(P0.05)健康鸭体内的C_(max),其他参数无显著性差异。氟苯尼考以30 mg·kg~(-1)体重肌内注射在健康和感染鸭体内具有吸收迅速,峰浓度高,体内分布广泛,消除较快的特点。
[Abstract]:[objective] to study and compare the pharmacokinetic characteristics of florfenicol injected intramuscularly in healthy and infected ducks at 2 weeks of age. [methods] 240 ducks were randomly divided into two groups: 120 ducks in each group. A single dose of florfenicol was injected intramuscularly with 30 mg kg 路kg ~ (-1) of body weight. The blood samples were collected for 45 min and 10 ~ 14 h before and after the administration of florfenicol at a single dose of 1.5g / kg ~ (-1) and 1.5mg / kg ~ (-1) 路L ~ (-1) 路kg ~ (-1) 路kg ~ (-1), respectively. The concentration of flufenicol in plasma was determined by high performance liquid chromatography (HPLC). The detection wavelength was 223 nm. The mobile phase consisted of acetonitrile and water. The volume ratio of acetonitrile and water was 26 and 74. The flow rate was 1 mL 路min -1. The chromatographic column was Agilent C186.mm 脳 150mm ~ (5) 渭 m ~ (-1). The amount of sample was 20 渭 L. The pharmacokinetic analysis software Win Nonlin 5.2.1 used non-atrioventricular model to deal with the plasma concentration and time data, and calculated the relevant pharmacokinetic parameters. The pharmacokinetic parameters were analyzed by SPSS17.0. [results] the peak concentration of florfenicol in healthy ducks was 22.88 卤3.11) 渭 g mL ~ (-1), the apparent distribution volume (VD / F) was 2.39 卤0.81L / kg ~ (-1), the body clearance rate was 0.64 卤0.11 L 路h ~ (-1) kg ~ (-1) ~ (-1), and the elimination of half life was (t ~ (12) 尾). (2.57卤0.51)h鍜岃嵂鏃舵洸绾夸笅闈㈢Н(AUC)涓，

本文编号：1964721