荷斯坦奶牛乳汁SCC与肝功酶、抗氧化及酮体的关系

发布时间：2018-06-02 10:58

本文选题：奶牛 + SCC　；参考：《广西大学》2015年硕士论文

【摘要】：奶牛乳汁中体细胞计数(SCC)不仅是检验乳汁质量的重要指标,也是监控乳房炎的重要手段,同时也是衡量奶牛健康状况的重要参数。本课题对广西某奶牛场的荷斯坦奶牛的产奶量及奶牛乳汁SCC进行检测,分析奶牛乳汁SCC的现状,与此同时,检测被检奶牛血浆肝功酶(AST、ALT、GGT)、乳清抗氧化指标(MDA、TAC)和乳清酮体水平,并分析奶牛乳汁SCC与肝功酶、抗氧化、酮体的相关性。(一)广西南宁地区某奶牛场乳汁体细胞调查荷斯坦奶牛,2014年7～10月份,随机采样,共425头测定其乳汁SCC含量,记录产奶量,分析乳汁SCC与产奶量之间的关系。结果:广西地区某奶牛场乳汁SCC的总体均值为54.08±131.99×104/mL,其中奶牛个体乳汁SCC小于等于20×104个/mL的奶样占被检总数的70.35%,小于50×104个/mL的占被检总数的80.45%,大于100×104个/mL的占被检总数的13.100%。该牛群产奶量从5、6月份开始下降,每年的6、7、8、9、10月份产奶量较低,处于均值以下。(二)乳汁SCC与肝功酶、酮体、抗氧化的关系研究本实验采用南宁地区某规模化养殖场,跟踪18头处于围产期的奶牛作为实验动物测定SCC、肝功酶、抗氧化能力、酮体相关指标。首先排除影响实验结果的个体动物,即酮病牛4头,使用水杨醛比色法测定实验动物血酮,高于1Omg/dL即剔除(试验期间某周血浆酮体含量一次或多次性超过限定值)。按试验过程中乳汁体细胞数大于50×104个/mL为标准将剩余14头实验牛分为7头高体细胞组和7头低体细胞组。结果显示:高体细胞组和低体细胞组肝功酶ALT、AST活性处在正常范围内,相关性不显著(P0.05)。低体细胞组中,肝功酶ALT与GGT差异显著(P0.05)。高体细胞组GGT活性大于低体细胞组,组间GGT差异显著(P0.05),高乳汁体细胞可能是导致肝功酶GGT活性上升的原因。乳汁SCC与抗氧化有一定的关系,高体细胞组乳汁SCC与M-MDA呈极显著负相关(r=-0.468,P0.01)。乳汁SCC与血酮、乳酮相关性不显著(P0.05),乳汁酮体与血浆酮体呈极显著正相关(r=0.508,P0.01)。
[Abstract]:The count of body cells in milk is not only an important index to test milk quality, but also an important means to monitor mastitis, and also an important parameter to measure the health status of dairy cows. In this paper, the milk yield and milk SCC of Holstein cows in a dairy farm in Guangxi were tested, and the present situation of milk SCC was analyzed. At the same time, the plasma liver function enzyme (ASTTAC), the antioxidant index of whey (MDATAC) and the level of whey ketone body were detected. The correlation between SCC and liver enzymes, antioxidants and ketones in milk of dairy cows was analyzed. (1) A survey of milk somatic cells in a dairy farm in Nanning, Guangxi. A total of 425 milk samples were randomly sampled in July and October 2014. The milk SCC content was measured, the milk yield was recorded, and the relationship between milk SCC and milk yield was analyzed. Results: the average value of SCC in a dairy farm in Guangxi was 54.08 卤131.99 脳 10 ~ 4 / mL. Milk samples with SCC less than 20 脳 10 ~ 4 / mL accounted for 70.35% of the total tested milk samples, 80.45% of the total milk samples were less than 50 脳 10 ~ (4) / mL / mL, and 13.100% of the total milk samples were more than 100 脳 10 ~ (4) / mL. The milk production of the herd began to decrease from May to June, and the milk production in October was lower than the average. (2) study on the relationship between milk SCC and liver enzyme, ketone body, antioxidation. In this experiment, 18 cows in perinatal period were followed up in a large scale farm in Nanning area to determine the relative indexes of SCC, liver function enzyme, antioxidant ability and ketone body. At first, four individual animals, that is, ketosis cattle, were excluded, and blood ketones of experimental animals were determined by salicylic aldehyde colorimetry, which was higher than that of 1Omg/dL (plasma ketone content once or several times exceeded the limited value during a week during the experiment). The remaining 14 cows were divided into 7 high somatic cells group and 7 low somatic cells group according to the standard of milk somatic cell number more than 50 脳 104 / mL. The results showed that the activity of alt AST in high somatic group and low somatic group was in the normal range, and the correlation was not significant (P 0.05). In the low somatic group, the difference of liver function enzyme ALT and GGT was significant (P 0.05). The activity of GGT in high somatic cell group was higher than that in low somatic cell group, and the difference of GGT between groups was significant (P 0.05). High milk somatic cells might be the cause of the increase of GGT activity of liver function. There was a certain relationship between milk SCC and antioxidant activity. In high somatic group, there was a significant negative correlation between SCC and M-MDA. There was no significant correlation between milk SCC and blood ketone, and there was no significant correlation between milk ketone body and plasma ketone body (P 0.05), but there was a very significant positive correlation between milk ketone body and plasma ketone body.
【学位授予单位】：广西大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823

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