融合肽hEGF-AWRK6对肉鸡生产性能及抗氧化和免疫机能的作用

发布时间：2018-06-04 01:24

  本文选题：肉鸡 + 融合肽hEGF-AWRK　； 参考：《沈阳农业大学学报》2017年03期

【摘要】：融合肽hEGF-AWRK6(EK)是项目组在前期研究工作中利用大肠杆菌重组表达系统,表达纯化得到的融合多肽,具有人表皮生长因子hEGF和抗菌肽AWRK6的双重功能。为探讨EK对AA肉鸡生产性能、肝脏抗氧化水平、免疫功能的影响,本研究选取84只1日龄健康AA仔肉鸡,随机分4组。对照组肉鸡饲喂基础日粮,试验Ⅰ组肉鸡饲喂添加50mg·kg~(-1)EK的基础日粮,试验Ⅱ组肉鸡饲喂添加100mg·kg~(-1)EK的基础日粮,抗生素组肉鸡饲喂添加5mg·kg~(-1)黄霉素的基础日粮,饲喂周期为42d。分别于试验的21d和42d,使用丙二醛(MDA)、超化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、总抗氧化能力(T-AOC)和鸡分泌型免疫球蛋白A(Sig-A)检测试剂盒,测定各组肉鸡相应指标的含量。结果表明:与对照组相比,饲喂添加EK的饲料显著提高了试验组肉鸡的体重、半净膛重与全净膛重,提高了肉鸡肝脏的SOD、GSH-PX活性水平,降低了MDA含量,同时肉鸡的免疫器官指数、巨噬细胞数量、脾脏细胞增殖能力和血清中Sig-A水平也显著提高。其中,试验组Ⅱ肉鸡的体重、半净膛重、全净膛重和肝脏抗氧化水平差异显著(p0.05),免疫器官指数和巨噬细胞的增殖能力差异极显著(p0.01)。EK对试验组肉鸡的半净膛率和全净堂率没有显著影响。融合肽EK能显著提高肉鸡的生长性能、肝脏抗氧化水平和免疫功能,具有替代饲料中抗生素的应用前景。
[Abstract]:The fusion peptide hEGF-AWRK6 (EK) is a recombinant expression system of Escherichia coli, which has dual functions of human epidermal growth factor (hEGF) and antimicrobial peptide (AWRK6). In order to study the effects of EK on the performance of AA broilers, liver antioxidant level and immune function, 84 1-day-old healthy AA broilers were randomly divided into 4 groups. The broilers in the control group were fed basic diet, the broilers in the first group were fed the basal diet supplemented with 50mg kg~(-1)EK, the broilers in the second group were fed the basic diet supplemented with 100mg kg~(-1)EK, and the broilers in the antibiotic group were fed the basal diet supplemented with 5mg KGG -1) for 42 days. Malondialdehyde (MDA) MDAA, supernatant dismutase (SOD), glutathione peroxidase (GSH-PXX), total antioxidant capacity (T-AOC) and secretory immunoglobulin A (Sig-An) were used to determine the content of corresponding indexes in broilers at 21d and 42d, respectively. The results showed that compared with the control group, the diet supplemented with EK significantly increased the body weight, half bore weight and the whole weight of the broiler, increased the activity of SOD GSH-PX in liver, decreased the content of MDA, and also increased the immune organ index of the broilers. The number of macrophages, the proliferation of spleen cells and the level of Sig-A in serum were also significantly increased. Among them, the body weight and half-bore weight of experimental group 鈪，

本文编号：1975183