表达耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响

发布时间：2018-06-04 01:31

本文选题：耐热肠毒素(STa) + 重组大肠杆菌　；参考：《中国畜牧兽医》2017年09期

【摘要】：试验旨在研究表达耐热肠毒素(STa)的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响。选取24头7日龄仔猪,随机分在4个日粮处理组,分别为对照组(人工乳),STa组(人工乳+2×10~9 CFU重组菌LMG194-pBAD-STa),LMG194组(人工乳+2×10~9 CFU大肠杆菌LMG194),K88组(人工乳+2×10~9 CFU大肠杆菌K88)。试验第5天进行攻毒,第7天屠宰取样,测量小肠黏膜的绒毛高度和隐窝深度,检测空肠、回肠、结肠及血清中的过氧化氢酶(CAT)、总一氧化氮合成酶(TNOS)与诱导型一氧化氮合酶(iNOS)的活力及血清中丙二醛(MDA)与过氧化氢(H_2O_2)的含量。试验结果表明,与对照组相比,STa组仔猪各肠段绒毛高度均显著降低(P0.05),十二指肠隐窝深度及绒毛高度与隐窝深度的比值显著降低(P0.05),十二指肠、空肠、回肠绒毛表面积显著降低(P0.05);同时,STa组回肠、结肠CAT活力显著降低,血清、空肠、回肠和结肠中的TNOS活力显著降低(P0.05),STa组结肠iNOS活力显著降低(P0.05),STa组血清中的MDA与H_2O_2含量显著提高(P0.05)。结果显示,表达STa的重组大肠杆菌可导致7日龄仔猪肠道结构损伤和抗氧化能力下降。
[Abstract]:The purpose of this study was to study the effects of recombinant Escherichia coli expressing STA on intestinal morphology and antioxidant function of 7 day old piglets. Twenty-four 7-day-old piglets were randomly divided into 4 groups: control group (artificial milk 2 脳 10 9 CFU recombinant strain LMG194-pBAD-STA LMG194), artificial milk 2 脳 10 9 CFU Escherichia coli LMG194K88 (artificial milk 2 脳 10 9 CFU Escherichia coli K88A). On the 5th day of the experiment, the virus was attacked, then slaughtered and sampled on the 7th day. The villus height and crypt depth of the intestinal mucosa were measured, and the jejunum and ileum were detected. The activities of catalase (CAT), total nitric oxide synthase (TNOS) and inducible nitric oxide synthase (iNOS) in colon and serum, and the contents of malondialdehyde (MDAs) and H _ 2O _ 2 in serum. The results showed that compared with the control group, the villus height of all intestinal segments in STA group was significantly lower than that of the control group, while the depth of duodenal recess and the ratio of villus height to crypt depth were significantly decreased in STA group, duodenum and jejunum. At the same time, the activity of CAT in the ileum, the activity of TNOS in serum, jejunum, ileum and colon were significantly decreased in the control group. The activity of iNOS in the colon of group P0.05 was significantly decreased. The contents of MDA and H_2O_2 in serum of group A were significantly increased. The results showed that recombinant Escherichia coli expressing STa could induce intestinal structure damage and decrease antioxidant ability in 7 day old piglets.
【作者单位】：武汉轻工大学动物营养与饲料科学湖北省重点实验室;中国水产科学研究院长江水产研究所农业部淡水鱼种质量监督检测中心;
【基金】：国家自然科学基金项目(31302089) 湖北省自然科学基金项目(2012FFBO4804)
【分类号】：S858.28

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