葡萄糖氧化酶对生长獭兔营养物质利用、盲肠菌群结构及肠道消化酶活性的影响

发布时间：2018-06-04 22:27

本文选题：葡萄糖氧化酶 + 营养物质利用　；参考：《动物营养学报》2017年02期

【摘要】：本试验旨在研究葡萄糖氧化酶(GOD)对生长獭兔营养物质利用、盲肠菌群结构及肠道消化酶活性的影响。选取40日龄生长獭兔150只,随机分为5组,每组设5个重复,每个重复6只(公母各占1/2)。对照组饲喂基础饲粮,4个试验组饲喂在基础饲粮的基础上分别添加0.1%、0.2%、0.3%和0.4%GOD的试验饲粮。预试期5 d,正试期30 d。结果表明:1)能量的表观消化率和表观消化能以对照组最低,0.4%组最高;各试验组粗纤维表观消化率较对照组有不同程度提高,其中0.4%组提高16.10%,差异显著(P0.05)。2)0.4%组可消化氮、氮沉积量、氮利用率和氮生物利用率分别较对照组提高12.39%、17.48%、15.68%和12.78%,且均差异显著(P0.05)。3)饲粮中添加GOD能够在一定程度上降低盲肠p H。与对照组相比,0.4%组盲肠乳酸菌和双歧杆菌数量显著提高(P0.05),大肠杆菌数量显著降低(P0.05);0.3%组盲肠双歧杆菌数量显著提高(P0.05)。4)0.4%组十二指肠部位胰蛋白酶活性显著高于对照组(P0.05),空肠部位乳糖酶活性极显著高于对照组(P0.01)。综合分析认为,饲粮中添加GOD能够改善饲粮中营养物质利用和獭兔肠道菌群结构,提高肠道消化酶活性,建议添加量为0.4%(60 U/kg)。
[Abstract]:The purpose of this study was to study the effects of glucose oxidase (GODO) on the utilization of nutrients, the structure of cecum flora and the digestive enzyme activity in the intestine of growing Rex rabbits. 150 40 day old growing Rex rabbits were randomly divided into 5 groups with 6 repeats in each group (male and female accounted for 1 / 2 / 2). The control group was fed with basic diet, and the four experimental groups were fed with 0.20.20.3wt% and 0.3wt% 0.4%GOD on the basis of basal diet respectively. The pre-test period was 5 days and the current test period was 30 days. The results showed that the apparent digestibility and apparent digestibility of energy were the highest in the control group (0.4%), the apparent digestibility of crude fiber in each experiment group was higher than that in the control group (16.10%). Nitrogen use efficiency and nitrogen bioavailability increased 12.39% and 12.78%, respectively, compared with the control group, and the difference was significant (P 0.05 路3) the addition of GOD in diet could reduce cecum pH to a certain extent. Compared with the control group, the number of cecal lactic acid bacteria and bifidobacterium in 0.4% group increased significantly, while the number of Escherichia coli decreased significantly. The number of bifidobacterium cecum in 0.3% group increased significantly the activity of trypsin in duodenum of 0.4% group was significantly higher than that in control group. The activity of lactase in jejunum was significantly higher than that in control group. The comprehensive analysis showed that dietary GOD could improve the nutrient utilization and intestinal flora structure of Rex rabbits, and increase the intestinal digestive enzyme activity. The recommended addition amount was 0.4 ~ 60 U / kg ~ (-1) 路kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1).
【作者单位】：河北农业大学山区研究所;河北省山区农业工程技术研究中心;河北农业大学动物科技学院;
【基金】：国家兔产业技术体系(CARS-44-05B) 河北农业大学青年科学基金(QN201302)
【分类号】：S829.1

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