抗氧化物质对提高家畜冻精受精能力的研究进展

发布时间：2018-06-06 00:22

本文选题：冻精 + 氧化应激损伤　；参考：《中国畜牧杂志》2017年08期

【摘要】：超低温冻存技术将低温生物学与生殖生物学紧密结合在一起,并在生命科学领域得到了广泛应用。在生殖生物学领域,配子与胚胎的超低温冻存有着广阔的研究前景。目前,常用于家畜繁殖育种的冷冻精液包括常规冻精液和性控冻精,精液经冷冻解冻,尤其是性控冻精在制备时经稀释、染料、激光、分离加压、冷冻以及受精时精液解冻等过程,均会产生大量活性氧(ROS)。ROS破坏精子内部抗氧化防御体系平衡,精子发生氧化应激损伤,造成精子质膜脂质过氧化(LPO)、DNA损伤、凋亡等,影响精子受精能力及受精卵的发育。内源性抗氧化剂不足,补充外源性抗氧化剂成为必然选择。近年来,抗氧化物质在家畜常规冻精中的研究日益增多,但在性控精液中研究很少。本文综述了番茄红素、抗坏血酸、白藜芦醇、褪黑素这几类抗氧化物质对降低家畜常规冻精及性控冻精氧化应激损伤作用机理的研究进展,以期筛选出高效的抗氧化剂以提高冻精质量。
[Abstract]:Cryopreservation technology combines cryogenic biology with reproductive biology and is widely used in the field of life science. In the field of reproductive biology, the cryopreservation of gametes and embryos has a broad prospect. At present, frozen semen, which is often used in breeding and breeding of domestic animals, includes conventional frozen semen and sex controlled semen. Semen is frozen and thawed, especially when the semen is prepared by dilution, dye, laser, separation and pressurization. During freezing and thawing of semen during fertilization, a large amount of reactive oxygen species (Ros) can destroy the balance of antioxidation defense system in spermatozoa, cause oxidative stress injury of spermatozoa, lead to DNA damage and apoptosis of sperm plasma membrane lipid peroxidation. It affects the fertilization ability of sperm and the development of fertilized eggs. The deficiency of endogenous antioxidants makes it an inevitable choice to supplement exogenous antioxidants. In recent years, the research of antioxidants in conventional frozen semen of domestic animals is increasing day by day, but it is seldom studied in sex-controlled semen. In this paper, the progress of research on the mechanism of lycopene, ascorbic acid, resveratrol and melatonin on reducing oxidative stress injury of conventional frozen spermatozoa and sex controlled frozen spermatozoa in domestic animals was reviewed. The aim of this study was to screen out effective antioxidants in order to improve the quality of frozen semen.
【作者单位】：中国农业科学院北京畜牧兽医研究所家畜胚胎工程与繁殖技术创新团队;
【基金】：资助项目:中国农业科学院“科研英才培育工程”;中国农业科学院科技创新工程(ASTIP-IAS06)
【分类号】：S814

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