7号染色体TEP1、CCNB1IP1、PARP2基因与公猪繁殖性状的关联性研究

发布时间：2018-06-06 02:26

本文选题：繁殖性状 + 位置候选基因　；参考：《江西农业大学》2015年硕士论文

【摘要】：公猪的繁殖性状是养猪生产的重要经济性状之一,但是该性状属于一种复杂的数量性状,遗传力低,而且受环境因素影响很大,常规育种手段难以取得有效的进展。近年来,随着分子生物学的不断发展,有不少研究者开始通过因果基因解析的途径来研究公猪繁殖性状的遗传机制,为种公猪的遗传改良和选育提供分子育种技术,同时也为男性繁殖障碍的研究与治疗提供重要参考。本实验室前期构建了白色杜洛克×二花脸资源家系群体,测定了F2代205头公猪的精液量、精子密度、总精子数、精子活力、睾丸重、附睾重、血浆睾酮浓度等繁殖性状;此外,前期研究通过基因型填补GWAS分析在7号染色体上定位到了影响300日龄公猪精子密度和总精子数的QTL区域,影响公猪精子密度及总精子数最显著相关的位点均为INRA0026647。本论文收集了356头杜洛克、长白和大白公猪的精液量、精子密度、总精子数和精子活力等性状,筛选了INRA0026647所处和相邻区域中的TEP1、CCNB1IP1和PARP2三个基因作为位置候选基因,研究这些基因的多态位点与F2公猪及商业公猪繁殖性状的关联性。结果表明,在F2群体中TEP1、PARP2和CCNB1IP1基因分别有9、11、10个SNP与公猪繁殖性状显著或极显著相关(P0.05或P0.01)。其中TEP1基因中TEP1-g.654 CA与精子密度的关联性最高(P=6.56E-08),并与精液量、总精子数、精子活力性状显著相关(P0.05)。PARP2基因中PARP2 g.107 AG(另有5个SNP位点与该位点完全连锁)与精子密度关联性最高(P=4.82E-06),与公猪精液量、总精子数显著相关(P0.05);PARP2 g.10568 AG与左附睾重、右附睾重极显著相关(P值分别为8.92E-04和6.14E-04)。CCNB1IP1基因中CCNB1IP1 g.14 CA(另有5个SNP位点与该位点完全连锁)与精子密度关联性最高(P=1.11E-06),并与精液量、总精子数、睾酮浓度显著相关(P0.05)。单倍型分析显示,TEP1有3个单倍型与精子密度、精液量、精子活力、附睾重显著或极显著相关(P0.05或P0.01);PARP2有5个单倍型与精子密度、精液量、附睾重、血浆睾酮浓度显著或极显著相关(P0.05或P0.01);CCNB1IP1有2个单倍型与精子密度显著相关(P0.05)。选择部分在F2群体中鉴别出的显著性SNP位点在商业群体中开展关联性分析,结果表明这些位点在商业群体中均表现出较低的最小等位基因型频率,各位点与公猪的精液品质性状没有显著关联(P0.05),可能的原因是样本量小、商业群体遗传背景的不一致及饲养管理条件的差异。q RT-PCR显示三个候选基因在猪14中组织中均有表达,且TEP1、PARP2基因在睾丸组织中有较高的表达水平。本研究为后期鉴别影响公猪繁殖性状的因果基因及公猪繁殖性状分子标记的开发提供了有价值的参考。
[Abstract]:The reproductive trait of boar is one of the important economic traits in pig production, but it belongs to a complex quantitative trait with low heritability and is greatly affected by environmental factors, so it is difficult to make effective progress in conventional breeding methods. In recent years, with the development of molecular biology, many researchers begin to study the genetic mechanism of reproductive traits of boars by means of causal gene analysis, and provide molecular breeding techniques for genetic improvement and breeding of boars. It also provides an important reference for the study and treatment of male reproductive disorders. A family population of white Duroc 脳 Erhualian resources was constructed in our laboratory. The reproductive characteristics of 205 F2 boars, such as sperm volume, sperm density, total sperm count, sperm motility, testicular weight, epididymal weight and plasma testosterone concentration, were measured. In the previous study, the QTL region affecting sperm density and total sperm number of 300-day-old boars was located on chromosome 7 by genotypic filling GWAS analysis. The most significant related loci to sperm density and total sperm count of boars were INRA0026647. In this paper, the spermatozoa, sperm density, total sperm count and sperm motility of 356 Duroc, Landrace and Landrace boars were collected, and three genes, TEP1CCNB1IP1 and PARP2, which were located in and adjacent regions of INRA0026647 were selected as position candidate genes. To study the association between the polymorphic loci of these genes and reproductive traits of F 2 boars and commercial boars. The results showed that in F2 population, the number of TEP1-PARP2 and CCNB1IP1 genes were 91, 11, respectively, and 10 SNP were significantly or extremely significantly correlated with reproductive traits of boars (P0.05 or P0.01). The correlation between TEP1-g.654 CA and sperm density was the highest in TEP1 gene. There was significant correlation between sperm motility traits and sperm density in PARP2 g.107AG107AGin P0.05N. PARP2 gene (5 other SNP loci were completely linked to this locus). The correlation between sperm density and spermatozoa concentration was significant. There was significant correlation between P0.05G105AG and left epididymal weight. The P value of the right epididymal weight was significantly correlated with the sperm density of 8.92E-04 and 6.14E-04).CCNB1IP1 genes CCNB1IP1 g.14CAA (5 other SNP loci were completely linked to this locus), and was significantly correlated with sperm volume, total sperm count and testosterone concentration (P 0.05). Haplotype analysis showed that there were three haplotypes associated with sperm density, sperm volume, sperm motility, epididymal weight, and epididymal weight. There were 5 haplotypes associated with sperm density, semen volume, epididymal weight, or P0.01 PARP2. Plasma testosterone concentration was significantly or extremely significantly correlated with P0.05P 0.05 or P0.01CCNB1IP1. Two haplotypes were significantly correlated with sperm density. Significant SNP loci identified in F2 populations were selected for association analysis in commercial populations. The results showed that these loci showed a lower minimum allele frequency in commercial populations. The results showed that there was no significant correlation between the semen quality traits of boars and the results of small sample size, inconsistent genetic background of commercial population and different breeding and management conditions. Q RT-PCR showed that all three candidate genes were expressed in pig 14. The expression level of TEP 1 and PARP2 gene in testicular tissues was higher. This study provides a valuable reference for the later identification of causal genes affecting reproductive traits and the development of molecular markers of reproductive traits in boars.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828

【相似文献】