禽呼肠孤病毒强毒株和弱毒疫苗株感染Vero细胞的蛋白质组学研究

发布时间：2018-06-06 02:42

本文选题：禽呼肠孤病毒 + 强毒株　；参考：《畜牧兽医学报》2016年02期

【摘要】：为研究在禽呼肠孤病毒(ARV)不同毒力毒株感染后不同时间点宿主细胞中蛋白质组变化,运用双向凝胶电泳技术和质谱鉴定的蛋白质组学方法,对ARV强毒株S1133、弱毒疫苗株aS1133感染Vero细胞和对照细胞在不同时间点的蛋白质样品进行检测。鉴定结果表明,共鉴定出44个差异表达蛋白质,这些蛋白质包括α-烯醇化酶(α-enolase)、过氧化物酶(Prx-4)、hnRNPs、微管蛋白(Tubulin)、蛋白磷酸酶、转录延伸因子等。GO软件分析显示这些差异蛋白质主要参与细胞信号、细胞骨架组成、能量代谢、核酸代谢、蛋白质折叠、细胞增殖及凋亡等生物学功能。荧光定量RT-PCR验证表明,hnRNP和Prx-4在S1133感染细胞中表达水平持续上调,而Tubulin和α-enolase仅分别在48和24h时表达上调,与双向凝胶电泳的结果一致。以上试验数据揭示了ARV不同毒力毒株感染后细胞中蛋白质表达的差异变化,有助于进一步阐明ARV和宿主之间的相互作用。
[Abstract]:In order to study the proteome changes in host cells at different time points after infection with different virulence strains of avian reovirus ARV, two dimensional gel electrophoresis and proteomics methods were used to identify ARV. The protein samples of Vero cells infected with ARV virulent strain S1133 and attenuated vaccine strain aS1133 and control cells at different time points were detected. The results showed that 44 differentially expressed proteins were identified, including 伪 -enolase (伪 -enolase), peroxidase (Prx-4), tubulin (tubulin), protein phosphatase (Phosphatase). Analysis of transcriptional extender and other .go software showed that these differential proteins were mainly involved in cell signal, cytoskeleton composition, energy metabolism, nucleic acid metabolism, protein folding, cell proliferation and apoptosis, and other biological functions. Fluorescence quantitative RT-PCR analysis showed that the expression of Tubulin and 伪 -enolase were up-regulated in S1133 infected cells, while the expression of Tubulin and 伪 -enolase were only up-regulated at 48 and 24 hours, respectively, which was consistent with the results of two-dimensional gel electrophoresis. The above experimental data revealed the difference of protein expression in the cells infected with different virulence strains of ARV, which was helpful to further clarify the interaction between ARV and host.
【作者单位】：广西壮族自治区兽医研究所广西畜禽疫苗新技术重点实验室;
【基金】：国家自然科学基金(31160512) 广西自然科学基金(2014GXNSFCA118006) 广西特聘专家专项(2011B020) 广西水产畜牧兽医局科技项目(桂渔牧科201452003)
【分类号】：S855.3

【相似文献】