绵羊TFAM基因编码蛋白生物信息学分析

发布时间：2018-06-06 23:52

  本文选题：线粒体转录因子A基因(TFAM) + 绵羊　； 参考：《基因组学与应用生物学》2017年02期

【摘要】：为进一步了解和研究绵羊线粒体转录因子A(TFAM)基因的结构和功能,采用生物信息学方法构建了不同物种的TFAM基因系统发育树,并对绵羊TFAM基因编码蛋白质理化性质、二级结构、亚细胞定位、蛋白磷酸化、三级结构及功能结构域等进行分析。结果表明:绵羊和山羊亲缘关系最为接近,其次是家牛,与原鸡、斑马鱼亲缘关系较远。绵羊TFAM基因共编码246个氨基酸,以赖氨酸含量最高;二级结构以α-螺旋和无规则卷曲占为主;综合分析,此蛋白为碱性、不稳定性亲水蛋白,不存在信号肽和跨膜结构域;其主要在细胞核、线粒体、细胞骨架内发挥其生物学作用。该蛋白的丝氨酸磷酸化位点比较多,而酪氨酸和苏氨酸的磷酸化位点相对较少;且含有2个HMG结构域,在两个HMG结构域之间存在一个由12个氨基酸残基组成的短连接区域。
[Abstract]:In order to further understand and study the structure and function of sheep mitochondrial transcription factor, TFAM gene phylogenetic tree of different species was constructed by bioinformatics, and the protein physicochemical properties and secondary structure of sheep TFAM gene were encoded. Subcellular localization, protein phosphorylation, tertiary structure and functional domains were analyzed. The results showed that the relationship between sheep and goat was the most close, followed by domestic cattle, and was far from the original chicken and zebrafish. Sheep TFAM gene encodes 246 amino acids with the highest content of lysine, and the secondary structure is mainly 伪 -helix and irregular curl, the protein is alkaline and unstable hydrophilic protein, and there is no signal peptide and transmembrane domain. It mainly plays its biological role in nucleus, mitochondria and cytoskeleton. The protein has more serine phosphorylation sites than tyrosine and threonine, and contains two HMG domains, and there is a short junction region composed of 12 amino acid residues between the two HMG domains.
【作者单位】： 河北省畜牧兽医研究所;河北农业大学动物科技学院;
【基金】：河北省科技支撑计划项目(15226308D)资助
【分类号】：S826;Q811.4
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本文编号：1988669