复方中药制剂对SD大鼠血清抗氧化功能的影响

发布时间：2018-06-07 12:20

本文选题：复方中药制剂 + SD大鼠　；参考：《浙江农业学报》2017年05期

【摘要】：由黄芪、板蓝根、炒白术、茯苓、陈皮、山楂、甘草和山药等中药制成的复方中药制剂,研究其对大鼠血清总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的影响。选择150只断奶SD大鼠(SPF级)分为对照组、1.5 mL·kg~(-1)组、3.0 mL·kg~(-1)组和6.0 mL·kg~(-1)组,对照组有15只大鼠,其余试验组各有45只大鼠。试验期间3个试验组大鼠按每千克体质量每天分别灌胃1.5、3.0和6.0 m L复方中药制剂(含生药1 g·m L~(-1)),对照组灌胃3.0 m L蒸馏水。于试验开始后10、30、60 d,每组分别取5只大鼠禁食禁水10 h后称重、心脏采血,分离血清,测定样本血清的T-AOC、T-SOD、GSHPx活性和MDA含量。结果表明,3.0 mL·kg~(-1)组和6.0 mL·kg~(-1)组在灌胃后10、30和60 d,其血清T-AOC活性与同日期对照组比较有显著(P0.05)或极显著(P0.01)增加;而血清中MDA含量显著(P0.05)或极显著(P0.01)减少。3.0 mL·kg~(-1)组在灌胃30和60 d,血清T-SOD活性与同日龄对照组比较显著增加(P0.05);6.0 mL·kg~(-1)组在灌胃30和60 d,血清GSH-Px活性与同日龄对照组比较显著增加(P0.05)。说明口服以黄芪、板蓝根、炒白术等药材组成的复方中药制剂可显著提高SD大鼠的抗氧化功能,其对大鼠的最适灌胃剂量为3.0 mL·kg~(-1)。
[Abstract]:Compound Chinese medicine preparations made from Chinese herbs such as Astragalus membranaceus, Radix Isatidis, Rhizoma Atractylodes, Poria cocos, Old Peel, Hawthorn, Glycyrrhiza and Chinese yam. To study the effects of TAOCX, T-SODX, GSH-Px and MDAs on serum total antioxidant capacity (TAOC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in rats. 150 SD rats with SPF grade were divided into control group (n = 15) and control group (n = 45). The rats were divided into control group (n = 15), control group (n = 15) and control group (n = 45). During the trial period, rats in the three experimental groups were gavaged with 1.5g / kg and 6.0 mL compound Chinese medicine preparations (including 1 g mL / L) and 3.0 mL / kg of distilled water, respectively, and the control group was given 3.0 mL distilled water per kg of body weight per day. Ten days after the beginning of the experiment, 5 rats in each group were weighed after fasting for 10 hours, blood was collected from the heart, serum was isolated, and the activity of T-AOCU T-SODX GSHPx and the content of MDA in the serum were measured. The results showed that the serum T-AOC activity was significantly increased in the 3. 0 mL / kg ~ (-1) and 6. 0 mL / kg ~ (-1) groups on the 30th and 60th day after gastric perfusion compared with the control group (P 0.05) or very significantly (P 0.01). However, the serum MDA content was significantly decreased (P0.05) or extremely significant (P0.01). The serum T-SOD activity was significantly increased at 30 and 60 days after gastric perfusion. Compared with the control group of the same age, the serum GSH-Px activity was significantly increased at 30 and 60 days after gastric perfusion, and the serum GSH-Px activity was significantly higher than that of the control group on the same day. The results showed that compound Chinese medicine preparation composed of Radix Astragali, Radix Isatidis and Radix Atractylodes could significantly improve the antioxidant function of SD rats, and the optimal dosage of oral administration was 3.0 mL kg 路kg ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1).
【作者单位】：安徽科技学院动物科学学院;安徽科技学院细胞与分子生物学研究中心;
【基金】：安徽省农业科技成果转化项目(1504032001) 安徽省科技攻关项目(1301042102) 安徽省高等学校自然科学研究重点项目(KJ2016A823) 安徽省第七批“115”产业创新团队项目(皖人才[2014]4号) 安徽科技学院重点建设学科项目(AKZDXK2015A04) 北京农学院兽医学(中兽医)北京市重点实验室开放课题(kf2016035)
【分类号】：S853.74

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