花生粕中胰蛋白酶抑制因子和皂苷含量调查

发布时间：2018-06-07 14:33

本文选题：花生粕 + 胰蛋白酶抑制因子　；参考：《饲料工业》2017年02期

【摘要】：研究旨在调查市售花生粕中胰蛋白酶抑制因子和皂苷的含量范围。采集山东省不同厂家的21批次花生粕,分别采用酶联免疫法试剂盒和比色法一酶标仪测定其内胰蛋白酶抑制因子与皂苷的含量。结果显示:花生粕中胰蛋白酶抑制因子P_5~P_(95)置信区间的含量范围为0.94~4.80 mg/g,平均含量为(2.41±1.21)mg/g,浸提与压榨工艺下花生粕中胰蛋白酶抑制因子的平均含量分别为(2.78±1.32)mg/g和(2.29±1.20)mg/g。花生粕中皂苷P_5~P_(95)置信区间的含量范围为5.78~9.01 mg/g,平均含量为(7.20±0.18)mg/g,浸提与压榨工艺下花生粕中皂苷平均含量分别为(6.63±0.82)mg/g和(7.38±1.38)mg/g。压榨法与浸提工艺下的花生粕相比,胰蛋白酶抑制因子含量低,皂苷含量高。同时发现,花生粕中胰蛋白酶抑制因子和皂苷含量之间无相关性,且不同花生粕中皂苷含量均高于胰蛋白酶抑制因子的含量。本研究将为饲料企业及研究者研究花生粕中的抗营养因子含量及其检测方法提供参考。
[Abstract]:The aim of this study was to investigate the content range of trypsin inhibitor and saponin in peanut meal. 21 batches of peanut meal from different manufacturers in Shandong Province were collected and the contents of trypsin inhibitor and saponins were determined by Elisa kit and colorimetric method. The results showed that the confidence interval of Peanut meal trypsin inhibitor P5 / PSP 95) was 0.94 卤4.80 mg / g, the average content was 2.41 卤1.21 mg / g, and the average content of trypsin inhibitor in peanut meal was 2.78 卤1.32)mg/g and 2.29 卤1.20 mg / g, respectively. The average content of saponin in peanut meal was 7.20 卤0.18 mg / g, and the average content of saponin in peanut meal was 6.63 卤0.82)mg/g and 7.38 卤1.38 mg / g, respectively. Compared with peanut meal, the content of trypsin inhibitor and saponins were lower and higher. It was also found that there was no correlation between trypsin inhibitor and saponin content in peanut meal, and the content of saponin in peanut meal was higher than that in trypsin inhibitor. This study will provide a reference for forage enterprises and researchers to study the content of anti-nutrition factor in peanut meal and its detection method.
【作者单位】：中国农业科学院饲料研究所;农业部食物与营养发展研究所;云南省兽药饲料检测所;
【基金】：国家公益性行业(农业)科研专项[201203015] 国家自然基金[21407176] 现代农业产业技术体系北京市家禽创新团队专项资金项目[CZ1108]
【分类号】：S816.4

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