石河子地区奶牛乳房炎源金黄色葡萄球菌的分离鉴定及耐药性分析

发布时间：2018-06-07 15:34

本文选题：石河子地区 + 奶牛　；参考：《黑龙江畜牧兽医》2017年02期

【摘要】：金黄色葡萄球菌是引起奶牛乳房炎的主要致病菌之一,为调查引起新疆石河子地区规模化奶牛场奶牛乳房炎中金黄色葡萄球菌的感染情况及其对常用药物的敏感性,于石河子地区部分奶牛场采集85份临床型或隐性乳房炎奶牛的奶样进行金黄色葡萄球菌的分离、鉴定及耐药性试验。结果表明:从85份奶样中分离到9种共计127株细菌,经选择性培养基培养、生化试验和16S rRNA PCR鉴定,其中60株为金黄色葡萄球菌,占47.24%;还有腐生葡萄球菌、表皮葡萄球菌、大肠杆菌、链球菌属、假单胞杆菌属、肠球菌属等。药敏试验结果表明:所有菌株对杆菌肽、青霉素、头孢氨苄和强力霉素高度耐药,耐药率分别为100%、71.67%、66.67%和76.67%;对氯霉素、氟苯尼考和阿米卡星的敏感率分别达80.00%、83.33%和66.67%;除此之外也有一些菌株对新霉素表现为既不明显耐药也不敏感现象。
[Abstract]:Staphylococcus aureus is one of the main pathogens causing mastitis in dairy cattle. In order to investigate the infection of Staphylococcus aureus in dairy cow mastitis in Shihezi area Xinjiang and its sensitivity to common drugs. A total of 85 milk samples of clinical or recessive mastitis cows were collected from some dairy farms in Shihezi area for the isolation, identification and drug resistance test of Staphylococcus aureus. The results showed that a total of 127strains of 9 species were isolated from 85 milk samples. They were identified by selective culture medium, biochemical test and 16s rRNA PCR. Among them, 60 strains were Staphylococcus aureus, accounting for 47.240.There were also staphylococcus saprophyticus, Staphylococcus epidermidis, Staphylococcus epidermidis, and Staphylococcus epidermidis. E. coli, Streptococcus, Pseudomonas, Enterococcus, etc. The results of drug sensitivity test showed that all strains were highly resistant to bacilli peptide, penicillin, cephalexin and doxycycline, and the resistance rates were 100% and 76.67%, respectively. The sensitivity rates of florfenicol and amikacin were 83.33% and 66.67%, respectively. In addition, some strains showed neither obvious resistance nor sensitivity to neomycin.
【作者单位】：石河子大学动物科技学院;
【基金】：国家自然科学基金项目(31560699) 新疆生产建设兵团应用基础研究计划项目(2015AG012;2015AG012);新疆生产建设兵团重点领域科技攻关计划项目(2013AB002)
【分类号】：S858.23

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