马鹿鹿茸不同部位差异蛋白质组学分析

发布时间：2018-06-08 07:47

本文选题：天山马鹿 + 鹿茸　；参考：《畜牧兽医学报》2017年08期

【摘要】：旨在从差异蛋白质组学角度为鹿茸生物学特性的阐明奠定理论基础。本研究以天山马鹿65和75d鹿茸不同部位为试验材料,通过双向凝胶电泳、MALDI-TOF-MS质谱鉴定技术,结合生物信息学分析,成功鉴定出65种差异蛋白质,涉及代谢过程、细胞过程、发育过程、定位以及应激等生物学过程,还参与了血小板活化、黏着斑、代谢通路、PI3K-Akt信号通路等代谢通路。这些蛋白包括骨发育相关蛋白4种、神经发育相关蛋白3种、血管发育相关蛋白2种、抗氧化、内质网应激相关蛋白4种、免疫相关蛋白7种、凋亡相关蛋白5种。结果显示,TTR、RBP4、CRABP1在由65d鹿茸过渡到75d鹿茸的过程中,对鹿茸生长发育起一定调控作用。PRDX2、TXNDC5、ERP29、Pdia6在内质网应激、抗氧化中起重要作用。内质网应激、内质网相关性死亡途径、线粒体凋亡途径可在一定程度上阐明鹿茸细胞快速增殖分化的同时存在大量细胞凋亡现象,以满足快速生长、发育的需要。另外,GMFβ、CATHL1两种蛋白在神经发育和炎症抗性中可能具有重要的作用。
[Abstract]:The aim is to lay a theoretical foundation for the elucidation of the biological characteristics of velvet antler from the perspective of differential proteomics. In this study, 65 different parts of velvet antler of Tianshan Wapiti were used as experimental materials. By means of two-dimensional gel electrophoresis (2-DE) MALDI-TOF-MS mass spectrometry and bioinformatics analysis, 65 differentially expressed proteins were successfully identified, which were involved in metabolic process and cellular process. The biological processes of development localization and stress are also involved in platelet activation adhesion and metabolic pathways such as PI3K-Akt signaling pathway. These proteins include 4 kinds of bone development related proteins, 3 kinds of neurodevelopmental related proteins, 2 kinds of vascular development related proteins, 4 kinds of antioxidation and endoplasmic reticulum stress-related proteins, 7 kinds of immune-associated proteins and 5 kinds of apoptosis-related proteins. The results showed that RBP4CRABP1 played an important role in regulating the growth and development of velvet antler from 65 d to 75 d. PRDX2TXNDC5 ERP29 Pdia6 played an important role in endoplasmic reticulum stress and antioxidation. Endoplasmic reticulum stress, endoplasmic reticulum associated death pathway and mitochondrial apoptosis pathway can clarify to a certain extent that a large number of apoptotic cells exist in the rapid proliferation and differentiation of velvet antler cells in order to meet the needs of rapid growth and development. In addition, GMF 尾 -CATHL1 proteins may play an important role in neurodevelopment and inflammatory resistance.
【作者单位】：中国农业科学院特产研究所特种经济动物分子生物学重点实验室;
【基金】：中国农业科学院科技创新工程“特种动物遗传资源创新团队”(CAAS-ASTIP-201X-ISAPS) 特种动物种质资源平台
【分类号】：S825

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