日粮能量水平和光照强度对獭兔繁殖性能和体况的影响

发布时间：2018-06-08 10:48

本文选题：能量 + 光照强度　；参考：《山东农业大学》2015年硕士论文

【摘要】：本试验旨在探讨不同能量水平的饲粮和不同强度的光照对周期性繁殖模式下獭兔繁殖性能和体况的影响。能量水平试验选用平均体重为(3.56±0.17)kg的经产獭兔150只,随机分成3组(每组50个重复,每个重复1只),采用42天繁殖模式,分别饲喂消化能为9.2、9.7和10.2 MJ/kg的饲粮。繁殖试验连续进行三胎,并在第一胎、第二胎产后15天静脉采血。结果如下:饲粮能量水平极显著影响母兔泌乳力(P=0.0062)、第一胎产后体重(P=0.0021)和仔兔断奶窝重(P=0.0006);显著影响第一胎(P=0.0475)和第三胎(P=0.0302)母兔泌乳21天体重。不同能量水平极显著影响血清肌酸激酶(P=0.0015)和甘油三酯(P=0.0013)含量,显著影响高密度脂蛋白胆固醇(P=0.0368)、总胆固醇(P=0.0476)和胰高血糖素含量(P=0.0159),对其他血清生化指标无显著影响(P0.05)。本试验研究结果表明,42天繁殖模式下獭兔适宜饲粮消化能水平为10.2 MJ/kg,高能量水平的日粮在周期性繁殖模式下可以提高母兔的繁殖性能,较好的维持母兔的体况。光照试验选用平均体重为(3.38±0.12kg)的后备母兔144只,随机分成3组(每组48个重复,每个重复1只),采用49天繁殖模式,分别给予不同强度的光照60lux(L),80lux(M),100lux(H)。相关的繁殖性能指标连续统计三胎,并在第二次配种前,每组选取6只母兔采集卵巢。结果表明:在这三种光照强度下母兔的繁殖性能,包括:受胎率、产仔数、活仔数、出生窝重、断奶窝重都没有显著的改变(P0.05)。对于卵巢内ER-α,FSHR,LHR,GnRHR-I,PGR基因的表达也没有显著影响(P0.05)。用免疫组化的方法检测卵巢内α-雌激素受体以及孕酮受体,发现光照强度对其分布与表达没有显著影响(P0.05)。但是在初始体重无显著差异的情况下,不同强度的光照对于母兔第一胎配种时的体重,第二胎配种和产仔时的体重有显著的影响(P0.05),在高光照强度下母兔体重增长缓慢。并且GHR基因的表达与体重变化出现相同的趋势。对于其他各个阶段(第一胎产后和泌乳21天,第二胎的泌乳21天以及第三胎)的体重均无显著影响(P0.05)。结果表明光照强度对母兔的繁殖性能没有显著的影响,但是强光照(100lux)在实验初期会影响母兔增重体重。
[Abstract]:The purpose of this study was to investigate the effects of different energy levels and different intensities of light on the reproductive performance and body condition of rex rabbits under periodic reproduction mode. One hundred and fifty rex rabbits with average body weight of 3.56 卤0.17)kg were randomly divided into 3 groups (50 replicates in each group and 1 repeats in each group), fed with a 42-day reproductive model and fed with a diet of 9.2g and 10.2 MJ / kg, respectively. Reproductive tests were carried out in three consecutive births, and the first and second postpartum venous blood collection 15 days. The results were as follows: the dietary energy level significantly affected the lactating capacity of female rabbits P0. 0062, the body weight of the first fetus P0. 0021) and the weaning litter weight of the litter P0. 0006, and significantly affected the lactating weight of the first and third embryos respectively. Different energy levels significantly affected the contents of serum creatine kinase P0. 0015) and triglyceride P0. 0013), high density lipoprotein cholesterol (HDL-C), total cholesterol (TC) 0. 0476) and glucagon (P0. 0159), but had no significant effect on other serum biochemical indexes. The results showed that the optimum dietary digestible energy level of rex rabbits was 10.2 MJ / kg under 42-day reproductive mode, and the high energy level diet could improve the reproductive performance of female rabbits and maintain the body condition of female rabbits. 144 female rabbits with an average weight of 3.38 卤0.12 kg were randomly divided into 3 groups (48 replicates in each group and 1 replica in each group). The related reproductive performance indexes were counted continuously, and the ovaries were collected from 6 female rabbits in each group before the second breeding. The results showed that the reproductive performance of female rabbits under these three light intensities, including conception rate, litter size, live litter size, birth litter weight and weaning litter weight, did not change significantly (P 0.05). There was also no significant effect on the expression of GnRHR-Ig PGR gene in ovarian ER- 伪 FSHR-LHR-LHR-LHR-LHR-P0.05. The 伪 -estrogen receptor and progesterone receptor in ovary were detected by immunohistochemical method. It was found that the distribution and expression of 伪 -estrogen receptor and progesterone receptor were not significantly affected by light intensity. However, under the condition of no significant difference in initial body weight, different light intensity had a significant effect on the body weight of the first and second born rabbits, and the body weight of the second and the second born rabbits, and the weight of the female rabbits increased slowly under high light intensity. The expression of GHR gene showed the same trend as weight change. There was no significant effect on the body weight of the other stages (the first and 21 days postpartum, the second and the third). The results showed that light intensity had no significant effect on the reproductive performance of female rabbits, but strong light lux100) would affect the weight gain of female rabbits at the beginning of the experiment.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S829.1

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