宁夏地区奶牛乳房炎病原菌分离鉴定及金葡菌毒力基因分析

发布时间：2018-06-08 17:16

本文选题：奶牛乳房炎 + 病原菌　；参考：《宁夏大学》2015年硕士论文

【摘要】：本研究以宁夏银川地区5个奶牛场、银南地区7个奶牛场、银北地区5个奶牛养殖场的94头临床型乳房炎奶牛、70头隐性乳房炎奶牛为实验对象。临床型乳房炎乳样共分离鉴定出40种159株病原菌,其中葡萄球菌41株(25.79%)、链球菌6株(3.78%)、其他G+球菌20株(12%)、G-杆菌74株(46%)、酵母菌18株(11.32%)。隐性乳房炎乳样共分离鉴定出33种共94株细菌,其中葡萄球菌28株(29.79%)、链球菌2株(2.13%)、其他G+球菌15株(15.96%)、G-杆菌45株(47.87%)、酵母菌4株(4.26%)。用15种抗细菌药敏片对临床型乳样检出率较高的12种细菌进行药敏试验。通过试验发现,引起奶牛乳房炎的病原菌种类呈现多样化、复杂化,与以往人们认识的引起乳房炎的三种主要细菌为金黄色葡萄球菌、链球菌、大肠杆菌存有较大差距,且多数感染均为两种或两种以上细菌的混合感染。引起乳房炎的细菌种类常随着地区的不同呈现不同的分布状态,即使同一地区,甚至同一奶牛场,致病菌的种类也会受操作规范、环境卫生等方面的影响。对宁夏地区140株隐性乳房炎金黄色葡萄球菌的nuc、Hla、Hlb、fnbp A、fnbp B及9种肠毒素共14种毒力基因进行检测。实验结果显示nuc、Hla、Hlb、fnbp A、fnbp B和SEI基因检出率高,SEA、SEB、SEC、SED、SEH、SEG、SEJ检出率较低或没有检出,可以提示nuc、Hla、Hlb、 fnbp A和SEI是宁夏地区隐性奶牛乳房炎金黄色葡萄球菌的主要毒力基因。
[Abstract]:In this study, 94 clinical mastitis cows with recessive mastitis were selected from 5 dairy farms in Yinchuan area, 7 dairy farms in south Yinnan area and 94 clinical mastitis cows in Yinbei area. A total of 40 species and 159 strains of pathogenic bacteria were isolated and identified from clinical mastitis, including 41 strains of Staphylococcus, 6 strains of Streptococcus, 20 strains of other G cocci, 74 strains of G- bacillus, and 18 strains of yeast, respectively. A total of 94 strains of 33 species were isolated from the milk samples of recessive mastitis, of which 28 strains were staphylococcus, 2 strains were streptococcus, 15 strains were isolated from 15 strains of G coccus, 45 strains were isolated from G- bacillus, and 4 strains were isolated from yeast. Fifteen antimicrobial drug sensitive tablets were used to test the drug sensitivity of 12 kinds of bacteria with high detection rate of clinical milk samples. It was found that the pathogenic bacteria causing mastitis of dairy cattle were diversified and complicated, which was different from the three main bacteria that caused mastitis were Staphylococcus aureus, Streptococcus aureus and Escherichia coli. And most of the infections were mixed infection of two or more kinds of bacteria. The species of bacteria causing mastitis often present different distribution with different regions. Even in the same area, even in the same dairy farm, the species of pathogenic bacteria will be affected by the operation standard, environmental hygiene and so on. A total of 14 virulence genes were detected in 140 strains of recessive mastitis Staphylococcus aureus in Ningxia. The results showed that the detection rate of Hla-Hla-Hlb-fnbp AfnBP B and SEI gene was high. The detection rate of SEDX SEDX SEHNGN SEJ was low or not, which suggested that nuclear Hlaa Hlb, fnbp A and SEI were the main virulence genes of recessive dairy cow mastitis Staphylococcus aureus in Ningxia area.
【学位授予单位】：宁夏大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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