宠物食品中肉毒杆菌LAMP检测方法的研究

发布时间：2018-06-08 23:52

  本文选题：肉毒杆菌 + LMAP技术　； 参考：《中国畜牧杂志》2017年04期

【摘要】：为研究宠物食品中肉毒杆菌环介导恒温扩增(LAMP)检测方法,本试验根据肉毒杆菌特异性基因片段(Ntn H)设计1组引物,应用LMAP技术,通过扩增肉毒杆菌和14种非肉毒杆菌基因片段,对建立的LAMP反应体系进行最佳温度及反应时间筛选试验、采用浊度法和染色法进行特异性试验以及与PCR对比的敏感性试验。结果表明:建立的LAMP反应体系只扩增肉毒杆菌基因,特异性良好;体系最佳反应温度为64℃,最佳反应时间为60 min;最低检测浓度为0.0001 pg/μL,比PCR高出10倍。本试验所建立的方法特异性好、灵敏度高,反应时间短,适用于宠物食品中肉毒杆菌的检测。
[Abstract]:In order to study the detection method of botulinum loop mediated isothermal amplification (Lampp) in pet food, a group of primers was designed according to the specific gene fragment of Botox and 14 kinds of non-botulinum gene fragments were amplified by LMAP technique. The optimum temperature and reaction time of the lamp reaction system were screened, the specificity test and the sensitivity test compared with PCR were carried out by turbidimetric method and staining method. The results showed that the lamp reaction system only amplified the Botox gene with good specificity, the optimum reaction temperature was 64 鈩，

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