腹泻仔猪生长性能和血液指标特征及灌服粪肠球菌对其影响

发布时间：2018-06-09 05:22

本文选题：哺乳仔猪 + 微生态制剂　；参考：《南京农业大学》2015年硕士论文

【摘要】：哺乳仔猪由于免疫系统、消化系统功能不完善,外界环境压力等原因常导致腹泻。而腹泻会对仔猪的生长性能和免疫系统产生重大影响。微生态制剂作为抗生素替代品,已开始应用于预防和控制仔猪腹泻,但其对仔猪的生长性能、腹泻率和血液指标的影响及其适宜剂量还需进一步研究。本文旨在研究,给腹泻和未腹泻的仔猪灌服不同剂量的粪肠球菌微生物制剂,观察其对仔猪的生长性能(主要是指仔猪日增重)和血液指标的影响。1腹泻仔猪血液常规、生化指标情况本实验选择胎次、产期、产仔数相近的新生仔猪48窝,第一阶段是在仔猪出生后,细心观察仔猪群腹泻状况,一旦发现某一窝有仔猪发生腹泻现象,立即将该腹泻仔猪及同窝同性别未腹泻的仔猪各1头,进行称重、屠宰、采集,直至采集20窝;第二阶段,将这20窝仔猪随机分对照组(C)、试验组1(T1)、试验组2(T2)、试验组3(T3)、试验组4(T4),每组4窝。五个组于腹泻当日(0d)、第3日(3d)、第6日(6d)全窝分别灌服双蒸水1 mL(C)、粪肠球菌2.5 × 108CFU/mL(T1)、5× 108CFU/mL(T2)、1 × 109CFU/mL(T3)、2 × 109CFU/mL(T4)的微生态制剂 1mL。到20日龄时,每窝选择试验期间发生过腹泻与同性别从未腹泻的仔猪各一头称重、采集样品分析腹泻仔猪血液常规、生化指标情况。结果表明:腹泻仔猪组血液总蛋白(TP)、球蛋白(GLO)均显著高于未腹泻组(P0.05);腹泻仔猪组血液常规指标与未腹泻仔猪组间无显著差异。2灌服微生态制剂对仔猪生长性能、腹泻率及血液指标的影响本实验研究灌服微生态制剂对仔猪日增重、腹泻率、血液常规以及血液生化指标的影响。结果表明:灌服高剂量微生态制剂组(T3组,1×109及T4组,2×109)仔猪平均日增重极显著高于对照组(P0.01)和低剂量微生态制剂组(P0.01),且灌服2 × 109的仔猪平均日增重极显著高于1 × 109组(P0.01),而低剂量组与对照组相比无差异。与对照组相比,灌服微生态制剂对血液球蛋白(GLO)和A/G均有显著影响(P0.05),其中灌喂高剂量微生态制剂的处理组4(T4)血液球蛋白(GLO)显著高于对照组C以及其他处理组(P0.05),灌喂高剂量微生态制剂的T4组和低剂量的T1组的A/G显著低于对照组(P0.05),且T4组中A/G显著低于T2、T3组;与对照组相比,各处理组血液尿素氮(BUN)的含量有下降的趋势(P0.1);腹泻对血液球蛋白(GLO)和A/G也有显著影响,腹泻组中血液球蛋白(GLO)显著低于未腹泻组,A/G值显著高于未腹泻组A/G。灌服微生态制剂对仔猪血常规指标无显著影响(P0.05)。
[Abstract]:Lactation piglets often suffer from diarrhea due to immune system, imperfect digestive system function and external environmental pressure. Diarrhea can have a significant impact on the performance and immune system of piglets. As a substitute for antibiotics, microecological agents have been used to prevent and control diarrhea in piglets. However, the effects of microecological agents on growth performance, diarrhea rate, blood index and their appropriate dosage of piglets need to be further studied. The purpose of this study was to study the effects of different doses of Enterococcus faecalis microorganism on growth performance (mainly daily gain of piglets) and blood index of diarrhea piglets. Biochemical parameters the experiment selected 48 litter of newborn piglets with similar birth order, delivery period and litter size. The first stage was to observe the diarrhea status of piglets carefully after the birth of piglets. Once a litter of piglets was found to have diarrhea phenomenon, the first stage was to observe the diarrhea status of piglets carefully after the birth of piglets. Immediately weighing, slaughtering and collecting the diarrhoea piglets and 1 piglet of the same litter and not having diarrhea of the same sex until the collection of 20 litter; the second stage, The 20 piglets were randomly divided into control group (n = 1), test group (n = 2), test group (n = 3) and control group (n = 4). The microecological agents of the five groups were 1 mL of double distilled water and 2.5 脳 10 8 CFU / mL T1, 5 脳 108 CFU / mLT2 / 1 脳 10 9 CFU / mLT3 / 2 脳 109 CFU / mLT4, respectively, in the whole nest on the day of diarrhea, 3 days on the 3rd day and 6 days after the 6th day of diarrhoea (n = 6), and the microecologic agent of Enterococcus faecalis was 2.5 脳 10 8 CFU / m L ~ (-1) T _ (1) / T _ (2) 脳 10 ~ (9) CFU / m ~ (L) T _ (4). At the age of 20 days, one piglet with diarrhea and one piglet of the same sex who had never had diarrhea occurred during each litter selection trial. The blood routine and biochemical indexes of the piglets with diarrhea were collected and analyzed. The results showed that the levels of serum total protein (TPN) and globulin (GLOL) in diarrhea piglets were significantly higher than those in non-diarrhea piglets (P 0.05), and there was no significant difference between diarrhea piglets and diarrhea piglets. 2. The growth performance of piglets treated with microecological preparations was not significantly different from that of diarrhea piglets. The effect of microecological preparation on daily gain, diarrhea rate, blood routine and blood biochemical indexes of piglets. The results showed that the average daily gain (ADG) of the high dose microecological preparation group was significantly higher than that of the control group (P 0.01) and the low dose microecological preparation group (P 0.01), and the average daily gain of the piglets treated with 2 脳 10 9 was significantly higher than that of the control group (P 0.01) and the average daily gain of the piglets treated with 2 脳 10 9 were significantly higher than that of the control group (P 0.01). There was no difference between the low dose group and the control group. Compared with the control group, The effects of microecological preparations on blood globulin (GLOL) and A / G ratio were significant (P 0.05). The blood globulin GLOs of the treatment group fed with high dose microecological preparations were significantly higher than those of the control group C and other treatment groups (P 0.05), and were fed with high dose microecology. The Ar / G of T4 group and T1 group were significantly lower than that of control group (P 0.05), and Ar / G of T4 group was significantly lower than that of T2T 3 group. Compared with the control group, the blood urea nitrogen (bun) content of each treatment group was decreased (P _ (0.1), and diarrhea had a significant effect on the blood globulin GLOs and A- / -G, and the blood globulin G _ (g) in diarrhea group was significantly lower than that in the non-diarrhea group (P _ (0.1), and the value of blood globulin G _ (g) in diarrhea group was significantly higher than that in non-diarrhea group. There was no significant effect of microecological preparation on blood routine index of piglets.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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