利用转录组测序技术分析ADC和NOS基因mRNA在鹅等级前卵泡中的表达

发布时间：2018-06-09 16:09

本文选题：籽鹅 + 转录组测序　；参考：《吉林农业大学学报》2017年04期

【摘要】：为研究精氨酸代谢的关键酶精氨酸脱羧酶(Arginine Decarboxylase,ADC)和一氧化氮合酶(Nitric-oxide Synthase,NOS)mRNA在鹅等级前卵泡中的表达情况。采用高通量测序技术对鹅等级前卵泡进行转录组测序分析,并通过荧光定量PCR对结果进行验证。通过FPKM值计算分析,这2个基因在鹅等级前卵泡中均有表达,且随着卵泡的发育,NOS mRNA的表达呈现先降低后增加再降低的趋势,在初级卵泡中的表达量达到最高值,在中白卵泡中的表达量最低。ADC mRNA的表达则呈现先增加后降低再增加的趋势,在初级卵泡中的表达量最低,在小白卵泡中的表达量最高。荧光定量PCR结果与转录组测序结果基本相同。
[Abstract]:To study the expression of arginine decarboxylase (Arginine DecarboxylaseADC) and nitric oxide synthase (nitric-oxide Synthase) mRNA in pregrade follicles of geese, we studied the expression of arginine decarboxylase (Arginine decarboxylase) and nitric oxide synthase (NOS) mRNA in goose follicles. The high throughput sequencing technique was used to analyze the transcriptional sequence of goose prefollicles, and the results were verified by fluorescence quantitative polymerase chain reaction (FQ-PCR). The results of FPKM analysis showed that these two genes were expressed in the pregrade follicles of geese, and the expression of NOS mRNA decreased first and then decreased with the development of follicles, and reached the highest level in the primary follicles. The expression of ADC mRNA was the lowest in the middle white follicles and then decreased, and the lowest in the primary follicles and the highest in the white follicles. The results of fluorescent quantitative PCR and transcriptome sequencing were basically the same.
【作者单位】：吉林农业大学动物科学技术学院;军事经济学院军需勤务指挥系;
【基金】：吉林农业大学首批青年拔尖人才支持计划项目(2016) 吉林省留学回国人员择优资助项目(2015) 吉林省科技厅重点科技攻关项目(20150204025NY) 国家科技部星火计化项目(2015GA660001) 吉林省牧业局项目(2016)
【分类号】：Q78;S835

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