体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱

发布时间：2018-06-10 03:11

本文选题：非淀粉多糖酶 + 生长猪　；参考：《动物营养学报》2017年04期

【摘要】：针对饲粮非淀粉多糖(NSP)酶的海量筛选工作和动物试验间的可比性差等问题,本研究探讨使用体外模拟法优化生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的NSP酶谱。首先采用单因素随机试验设计,研究NSP酶的添加水平与饲粮体外干物质消化率(IVDMD)的关系。在玉米-豆粕型饲粮和玉米-杂粕型饲粮中分别添加不同水平的纤维素酶、木聚糖酶、β-葡聚糖酶、β-甘露聚糖酶、α-半乳糖苷酶和果胶酶6种NSP酶,分析各NSP酶对饲粮IVDMD的作用效果。然后采用二次回归旋转正交组合试验设计,筛选2种饲粮中6种NSP酶的最佳酶谱。结果表明:1)6种NSP酶的添加水平与2种类型猪饲粮IVDMD之间存在二次曲线关系。2)α-半乳糖苷酶对玉米-豆粕型饲粮的IVDMD提升最高,达到了1.28%,木聚糖酶对玉米-杂粕型饲粮的IVDMD提升最高,达到了1.95%。3)玉米-豆粕型饲粮的最佳酶谱为:纤维素酶533.6 U/kg、木聚糖酶9 983.7 U/kg、β-葡聚糖酶1 014.4 U/kg、β-甘露聚糖酶4 080.6 U/kg、α-半乳糖苷酶251.6 U/kg和果胶酶107.3 U/kg。玉米-杂粕型饲粮的最佳酶谱为:纤维素酶960.0 U/kg、木聚糖酶17 177.6 U/kg、β-葡聚糖酶405.8 U/kg、β-甘露聚糖酶19 023.2U/kg、α-半乳糖苷酶307.2 U/kg和果胶酶96.9 U/kg。4)优化后的酶谱使玉米-豆粕型饲粮的IVDMD提升了3.26%,使玉米-杂粕型饲粮的IVDMD提升了3.75%。由此可见,6种NSP酶联合使用能够更大程度地提高生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的IVDMD。
[Abstract]:In view of the problems of mass screening of non-starch polysaccharide (NSP) enzymes in diet and poor comparability among animal experiments, the in vitro simulation method was used to optimize the NSP zymogram of corn-soybean meal diet and corn-hybrid meal diet. The relationship between dietary dry matter digestibility and in vitro dry matter digestibility (DMD) was studied by using a single factor randomized trial design to study the relationship between the level of NSP enzyme supplementation and dietary dry matter digestibility in vitro. Different levels of cellulase, xylanase, 尾 -glucanase, 尾 -mannanase, 伪 -galactosidase and Pectinase were added to corn-soybean meal diet and corn-hybrid meal diet, respectively. The effects of NSP enzymes on dietary IVDMD were analyzed. Then the optimum zymogram of 6 kinds of NSP enzymes in 2 diets was selected by quadratic regression rotation orthogonal combination design. The results showed that there was a conic relationship between the addition level of 6 NSP enzymes and IVDMD in two types of pig diets. 2) 伪 -galactosidase increased the IVDMD of corn-soybean meal diet the highest. At 1.28%, Xylanase had the highest increase in IVDMD of corn-mixed meal diet, The optimum zymogram of corn soybean meal diet was as follows: cellulase 533.6 U / kg, xylanase 9 983.7 U / kg, 尾 -glucanase 1 014.4 U / kg, 尾 -mannanase 4 080.6 U / kg, 伪 -galactosidase 251.6 U / kg and Pectinase 107.3 U / kg. The optimum zymogram of corn-mixed meal diet was as follows: cellulase 960.0 U / kg, xylanase 17 177.6 U / kg, 尾 -glucanase 405.8 U / kg, 尾 -mannanase 19 023.2Ukg, 伪 -galactosidase 307.2 Ukg and Pectinase 96.9 U / kg 路4). IVDMD increased by 3.26%, and increased by 3.75% in corn-mixed meal diet. It can be seen that the combined use of six NSP enzymes can improve the IVDMDs of growing pig corn-soybean meal diet and corn-hybrid meal diet to a greater extent.
【作者单位】：中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;中国农业科学院淮安健康养殖与饲料研究中心;江苏华威农牧发展有限公司;
【基金】：国家科技支撑计划项目课题(2012BAD39B01) 中国农业科学院科技创新工程(ASTIP-IAS07) 产学研联合创新资金(BY2013054) 苏北科技发展计划(BN2014034)
【分类号】：S828.5

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