鸡血藤总黄酮对猪圆环病毒2型感染小鼠脾脏氧化应激的影响

发布时间：2018-06-10 03:30

  本文选题：鸡血藤总黄酮 + 猪圆环病毒型(PCV)　； 参考：《中国畜牧兽医》2017年05期

【摘要】：试验旨在探讨鸡血藤总黄酮(TFSD)对猪圆环病毒2型(PCV2)感染小鼠脾脏氧化应激的影响。将70只昆明小鼠随机分为7组,对照组、TFSD组(100mg/kg体重)、PCV2组、PCV2+维生素C(VC)组、PCV2+不同浓度TFSD(25、50和100mg/kg体重)组,每组10只,连续3d采用灌胃和腹腔注射PCV2病毒液的方法建立小鼠氧化应激模型,第4~6天每天按上述分别灌胃给予生理盐水、VC或TFSD。第7天剖杀小鼠,取脾脏分析黄嘌呤氧化酶(XOD)、髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)活力,并检测还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)水平,计算GSH/GSSH。结果显示,PCV2感染小鼠后,脾脏中XOD与MPO的活力及GSSG水平显著上升(P0.05),SOD活力、GSH水平及GSH/GSSG显著下降(P0.05)。TFSD处理小鼠脾脏的SOD活力、GSH水平和GSH/GSSG比值均显著高于PCV2组(P0.05),而XOD、MPO活力和GSSG水平则显著低于PCV2组(P0.05),对PCV2引起的氧化应激相关酶活力与相关分子水平变化的抑制作用优于VC。结果表明,鸡血藤总黄酮对PCV2诱导的小鼠脾脏氧化应激有良好的调节作用。
[Abstract]:The purpose of this study was to investigate the effect of TFSD on oxidative stress in spleen of mice infected with porcine circovirus type 2 (PCV2). 70 Kunming mice were randomly divided into 7 groups. The control group was divided into two groups: the control group (100 mg / kg BW) and the PCV2 vitamin C (VC2) group (10 rats in each group) with different concentrations of TFV2, TFSDD, 2550 and 100mg/kg body weight (n = 10 in each group), and 10 mice in each group were divided into two groups, the control group (n = 10) and the control group (n = 10). The oxidative stress model of mice was established by intraperitoneal injection of PCV2 virus solution and intragastric perfusion for 3 consecutive days. The mice were given normal saline VC or TFSD respectively daily on the 4th day of 6 days. On the 7th day, the mice were dissected and the activities of XODO, MPO and SOD were analyzed, and the GSH) and GSSGG levels of GSH and GSSG were measured. The GSH / GSSH was calculated. The results showed that PCV2 infected mice, The activities of XOD, MPO and GSSG in spleen increased significantly. The activity of SOD and GSH of GSH / GSSG decreased significantly in the spleen of mice treated with P0.05. TFSD. The activity of SOD and the ratio of GSH / GSSG in spleen were significantly higher than those in group PCV2, while the activity of XODMPO and the level of GSSG in PCV2 group were significantly lower than those in group PCV2. The inhibitory effect of P0.05 on the activity of oxidative stress-related enzymes and the changes of related molecules induced by PCV2 was superior to that of VC. The results showed that total flavone could regulate the oxidative stress induced by PCV2 in mice.
【作者单位】： 广西大学动物科学技术学院;
【基金】：广西自然科学基金(2014GXNSFCA118019) 广西教育厅科研项目(KY2015ZD007) “大学生创新创业训练计划”创新训练项目(201610593081)
【分类号】：S853.7
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本文编号：2001823