樱桃谷肉鸭源细小病毒QH-L01株的分离鉴定及VP1基因序列分析

发布时间：2018-06-10 10:23

本文选题：樱桃谷肉鸭 + 细小病毒　；参考：《中国兽医科学》2017年07期

【摘要】：2016年4月,四川邛崃某鸭场送检30只樱桃谷肉鸭,临床表现鸭喙变短、舌头伸出。从送检鸭脏器中分离鉴定出1株鸭源细小病毒,命名为QH-L01株。为进一步了解该病毒毒力及分子生物学特性,对其进行ELD50测定、VP1基因测序,并与其他细小病毒毒株进行比较。ELD50测定结果为10-6.54ELD50/0.2 m L;VP1核苷酸和氨基酸序列分析结果表明,该病毒结构基因全长2 203 bp,编码734个氨基酸,与鹅细小病毒核苷酸同源性为92.8%~99.8%,与番鸭细小病毒同源性为77.4%~87.3%;VP1编码的氨基酸同源性和鹅细小病毒及番鸭细小病毒分别为95.2%~99.5%和85.4%~91.1%。VP1基因进化分析显示,该病毒和鹅细小病毒SYG26-35分离株处于同一进化分支上。动物回归试验结果显示,试验组出现明显的鸭源细小病毒BADS症状,对照组无明显变化,表明该病毒分离鉴定成功。
[Abstract]:In April 2016, 30 Cherry Valley ducks were examined in Qionglai duck farm, Sichuan province. A strain of duck parvovirus, named QH-L01 strain, was isolated and identified from duck viscera. In order to further understand the virulence and molecular biological characteristics of the virus, the VP1 gene was sequenced by ELD50, and compared with other parvovirus strains, the results were 10-6.54 ELD50 / 0.2 mL VP1 nucleotide and amino acid sequence analysis. The structural gene of the virus is 2 203 BP and encodes 734 amino acids. The nucleotide homology with goose parvovirus was 92.8%, with muscovy duck parvovirus 77.4%, and with goose parvovirus and muscovy duck parvovirus 95,299.5% and 85.4% 91.1%, respectively. The results of phylogenetic analysis showed that the amino acid homology of VP1 was 77.4% and that of goose parvovirus and muscovy duck parvovirus were 95.2% and 91.1%, respectively. The virus is in the same evolutionary branch as the goose parvovirus SYG 26-35 isolate. The results of animal regression test showed that there were obvious symptoms of duck parvovirus Bads in the test group, but there was no obvious change in the control group, which indicated that the virus was isolated and identified successfully.
【作者单位】：四川农业大学动物医学院动物疾病模型实验室;四川农业大学预防兽医研究所;
【基金】：国家科技支撑计划课题(2014BAI03B01) 国家重大科学仪器设备开发专项(2013YQ49085906)
【分类号】：S852.65

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