大通牦牛心肌低氧适应的组织学特点

发布时间：2018-06-12 12:19

本文选题：大通牦牛 + 心肌　；参考：《中国兽医学报》2017年03期

【摘要】：选取两个海拔高度大通牦牛作为研究对象,以乐都地区牦牛为对照,利用光镜技术和计算机图像分析系统测定心肌肌纤维直径、表面积密度;利用透射电镜技术比较心肌线粒体的平均体积(V)、体积密度(VV)和面数密度(NA)等结构参数;利用免疫组织化学技术对心肌细胞血管内皮生长因子(VEGF)和微血管密度(MVD)进行检测,并对以上数据进行相关性分析。结果显示:(1)大通牦牛心肌肌纤维直径均细于乐都地区牦牛心肌肌纤维直径,肌纤维表面积密度均高于乐都地区牦牛,且三者两两比较差异显著(P0.05);(2)大通牦牛的VEGF和MVD均高于乐都地区牦牛,差异显著(P0.05);(3)大通牦牛心肌线粒体的V和VV均大于乐都地区牦牛,差异显著(P0.05);海拔3 700m的大通牦牛心肌线粒体面数密度大于海拔3 200m的乐都地区牦牛,差异显著(P0.05)。结果表明,大通牦牛心肌组织主要表现为心肌纤维直径细、表面积密度大、心肌组织血管内皮生长因子和微血管密度大的遗传学特点。而心肌线粒体的低氧适应表现为,随着海拔高度的增加,心肌线粒体平均体积增大,面数密度和体密度增加的特点。
[Abstract]:The diameter and surface density of cardiac muscle fiber were measured by light microscope and computer image analysis system. The structural parameters of myocardial mitochondria were compared by transmission electron microscopy (TEM), mean volume density (VV) and surface number density (NAA), and vascular endothelial growth factor (VEGF) and microvessel density (MVD) were detected by immunohistochemistry. And carries on the correlation analysis to the above data. The results showed that the diameter of cardiac muscle fiber in Datong yak was smaller than that in Ledu area, and the density of muscle fiber surface area was higher than that in Ledu area. The difference of VEGF and MVD of Datong yak was higher than that of Yak in Ledu area, and the difference was significant (P 0.05). The V and VV of myocardial mitochondria of Datong yak were higher than that of Yak in Ledu area. The difference was significant (P 0.05), and the density of mitochondria surface of Datong yak at 3 700 m above sea level was higher than that of Yak in Ledu area at 3 200 m above sea level, the difference was significant (P 0 05). The results showed that the myocardial tissue of Datong yak was characterized by fine diameter of myocardial fiber, large density of surface area, high vascular endothelial growth factor (VEGF) and microvessel density. However, the hypoxic adaptation of myocardial mitochondria was characterized by the increase of the average volume of myocardial mitochondria, the increase of surface number density and volume density with the increase of altitude.
【作者单位】：青海大学农牧学院;
【基金】：国家自然科学基金资助项目(31460643) 青海省科技厅资助项目(2013-Z-713)
【分类号】：S823.85

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