猪源大肠埃希菌超广谱β-内酰胺酶的检测及其耐药性分析

发布时间：2018-06-12 15:05

本文选题：大肠埃希菌 + 超广谱β-内酰胺酶　；参考：《河南农业科学》2017年02期

【摘要】：为了解辽宁地区猪源大肠埃希菌产超广谱β-内酰胺酶(ESBLs)情况及耐药情况,采用纸片扩散法对从辽宁地区分离到的65株大肠埃希菌进行ESBLs检测及耐药性分析,并用PCR方法对确证的产ESBLs大肠埃希菌进行TEM、CTX-M、OXA和SHV基因型检测。结果发现,在65株大肠埃希菌中,20株大肠埃希菌产ESBLs,阳性率为30.77%;在20株产ESBLs大肠埃希菌中,9株为TEM型,1株为CTX-M型,8株为同时携带TEM型和CTX-M型,1株为同时携带TEM型和OXA型,1株为同时携带TEM型、CTX-M型和OXA型。药敏试验结果表明,产ESBLs菌株的耐药率明显高于非产ESBLS菌株,且产ESBLs菌株均为多重耐药菌株。可见,辽宁地区产ESBLs猪源大肠埃希菌的检出率较高,流行的主要基因型为TEM型和TEM+CTX-M型。
[Abstract]:In order to understand the extended-spectrum 尾 -lactamases (ESBLs) production and drug resistance of Escherichia coli isolated from Liaoning Province, 65 strains of Escherichia coli isolated from Liaoning were detected by disk diffusion method and their drug resistance was analyzed. The ESBLs-producing Escherichia coli (ESBLs-producing Escherichia coli) was detected for TEMX-MX-OXA and SHV genotypes by PCR. And it turns out, Among 65 strains of Escherichia coli, 20 strains of Escherichia coli produced ESBLswith a positive rate of 30.77, and 9 of 20 strains of ESBLs-producing Escherichia coli were Tem type 1 strain, CTX-M type 8 strain, carrying Tem type and CTX-M type 1 strain simultaneously carrying TEM type and OXA type 1 strain of ESBLs-producing Escherichia coli, and of 20 strains of ESBLs-producing Escherichia coli, 9 strains were Tem type and 1 strain of OXA type. Tem type CTX-M and OXA type were carried simultaneously. The results of drug sensitivity test showed that the resistance rate of ESBLs-producing strains was significantly higher than that of non-ESBLs-producing strains, and ESBLs-producing strains were multidrug resistant strains. It can be seen that the detection rate of ESBLs-producing Escherichia coli was high in Liaoning province, and the main genotypes were TEM and TEM CTX-M.
【作者单位】：辽宁省动物疫病预防控制中心;
【基金】：辽宁省自然科学基金项目(201402573,2130106)
【分类号】：R440

【参考文献】