提高荷斯坦牛移植日本黑和牛胚胎受胎率的研究

发布时间：2018-06-12 15:26

  本文选题：荷斯坦牛 + 移植　； 参考：《石河子大学》2017年硕士论文

【摘要】：本研究在生产实践基础上,针对荷斯坦青年牛作为受体提高胚胎移植受胎率的技术要点,在新疆西部牧业中心奶牛场和新疆石河子市新安镇双锵牧业有限责任公司分别进行长效FSH与常规FSH超排效果的对比分析、发情配种后第7天黄体质量监测与血清激素的检测、胚胎移植主要程序的操作。应用直肠检查和B超对超排实验牛和受体牛进行跟踪检测和筛查,并对受体牛实施移植后优化化保胎方案。结合发情配种后第7天黄体直径、周长、面积、体积与血清中E2、LH、P4三种激素水平的变化规律,为今后进一步提高荷斯坦青年牛为受体移植日本黑和牛胚胎受胎率奠定一定基础。本研究在西部牧业良繁中心奶牛场选择18头淘汰奶牛,其中9头采用4天8针减量注射法注射短效的FSH,另外9头一针注射的长效PVP-FSH,通过B超诊断和屠宰后的观察。超排效果明显,卵泡上有很多的排卵点,结果表明,短效FSH方案卵泡数量总计为179个,平均19.8个;排卵点为82个,平均9.1个;长效PVP-FSH超排方案,卵泡总计248个,平均为27.5个;排卵点为112个,平均12.4个。两者效果差异不显著(P0.05)。为了研究奶牛发情后第7天卵巢黄体周长、面积、体积和直径与血清中激素水平的关系进行研究,随机对85头奶牛进行B超检查,对血清中P4、E2和LH用ELISA方法进行激素水平检测。以便完善在胚胎移植操作程序中对受体牛选择。结果显示,在检查的85头奶牛中有16头无黄体,把无黄体的奶牛设为对照组,把有黄体的奶牛按照不同的指标分组分析黄体直径、面积、周长、体积和血清P4、E2和LH水平的相互关系。发情配种后第7天通过B超监测黄体直径、面积、周长、体积和ELISA血清P4、E2和LH水平的相互关系。结果显示,配种3次以上组与配种3次以下组,黄体直径、面积、周长、体积和BCS之间差异不显著(P0.05),但产后天数之间差异显著(P0.05);配种1次与配种2次黄体直径指标之间差异显著(P0.05);体况评分各组黄体指标之间差异均不显著(P0.05),不同黄体直径组2.6cmCLD≤3cm组和CLD≥3cm组E2和P4水平差异显著(P0.05);其它各组之间不同黄体体积、面积和周长指标E2、LH、P4水平差异均不显著(P0.05)。为了获得更多的纯种和牛,选择199头牛采用CIDR埋栓做同期发情,发情率为96%(191/199);采用临床观察、直肠检查和B超检查筛选出113头荷斯坦牛作为受体牛,在发情后第7天用B型超声仪对受体牛黄体直径进行测量,对符合条件的受体牛进行胚胎移植,将不同黄体直径分为3个组,分别为1cm≤CLD≤1.5cm、1.5cmCLD≤2.5cm和CLD2.5cm。在移植后第23天用B超做妊娠诊断,以便研究黄体直径对移植受胎率的影响。结果表明,黄体直径1cm≤CLD≤1.5cm组、1.5cmCLD≤2.5cm组、CLD2.5cm组受胎率分别是42.86%(6/14)、38.46%(25/65)和44.44%(12/27),各组之间差异均不显著(P0.05)。
[Abstract]:Based on the production practice, this study aimed at the key points of improving the conception rate of embryo transfer in Holstein young cattle as a receptor. The results of long-term FSH and routine FSH superovulation were compared and analyzed in the dairy farm in the west of Xinjiang and Shuangqiang Animal Husbandry Co., Ltd., Xinan Town, Shihezi City, Xinjiang. The quality of luteal body and serum hormone were monitored 7 days after oestrus mating. The operation of the main procedure for embryo transfer. Rectal examination and B-ultrasound were used to detect and screen the superovulation test and recipient cattle, and the recipient cattle were optimized for fetal preservation after transplantation. The changes of luteal body diameter, circumference, area, volume and serum E2LHHHP4 levels on the 7th day after oestrus mating laid a foundation for further improving the embryo conception rate of Japanese black and black cattle embryos transferred by Holstein young cattle as recipients in the future. In this study, 18 dairy cows were selected for elimination in West China Livestock breeding Center. Among them, 9 cows were injected with short-effect FSHs with 4 days and 8 injections, and 9 cows with long-acting PVP-FSHs were injected with one needle. The results were diagnosed by B-ultrasound and observed after slaughter. The results showed that the total number of follicles was 179 (mean 19.8), the ovulation points were 82 (average 9.1), and the long-acting PVP-FSH superovulation scheme had 248 follicles with an average of 27.5. The number of ovulation points was 112, with an average of 12.4. There was no significant difference in effect between the two groups (P 0.05). In order to study the relationship between the peripheral length, area, volume and diameter of corpus luteum and serum hormone level in 85 cows after estrus 7 days after estrus, 85 cows were examined by B ultrasound, and serum P4E 2 and LH were detected by Elisa. In order to improve the selection of recipient cattle in the procedure of embryo transfer. The results showed that there were 16 lute-free cows in the 85 cows examined, and the luteinless cows were divided into two groups according to different indexes to analyze the diameter, area and circumference of luteal body. The relationship between volume and serum P _ 4 E _ 2 and LH levels. The relationship between the diameter, area, circumference and volume of corpus luteum and the serum levels of P4E _ 2 and LH in Elisa was monitored by B-ultrasound on the 7th day after oestrus mating. The results showed that the diameter, area and circumference of corpus luteum were higher than 3 times and less than 3 times. There was no significant difference between volume and BCS, but there was significant difference between postpartum days (P 0.05), there was significant difference between the two indexes of luteal body diameter (P 0.05), and there was no significant difference in luteal body index among all groups in body condition score (P 0.05), but there was no significant difference among different luteal bodies. There were significant differences in E2 and P4 levels between the diameter group (2.6 cm CLD 鈮，

本文编号：2010139