日粮添加亮氨酸对宫内发育迟缓断奶仔猪小肠葡萄糖吸收和能量代谢的影响

发布时间：2018-06-14 16:04

本文选题：亮氨酸 + 宫内发育迟缓　；参考：《南京农业大学学报》2017年02期

【摘要】：[目的]本文旨在研究亮氨酸对宫内发育迟缓(IUGR)断奶仔猪小肠葡萄糖吸收和能量代谢的影响。[方法]从正常分娩的16头母猪中选择正常初生体质量(NBW)和IUGR新生仔猪各16头,于14日龄断奶,分别饲喂基础日粮(含1.45%亮氨酸)或亮氨酸日粮(含1.80%亮氨酸)并随机分为4组,即NC(NBW+基础日粮)、NL(NBW+亮氨酸日粮)、IC(IUGR+基础日粮)、IL(IUGR+亮氨酸日粮),每组8头,公母各半,35日龄屠宰取样,对空肠酶活性和相关酶mRNA表达量进行测定。[结果]IUGR仔猪空肠AKP、Na~+/K~+-ATP酶、PK和线粒体MDH活性及ATP含量均显著下降(P0.05),SGLT1和PK mRNA的表达量有下降的趋势,而AMPK-α1和mTOR mRNA的表达量显著下降(P0.05);日粮添加亮氨酸促使IUGR仔猪空肠AKP及线粒体MDH和SDH活性显著升高(P0.05),Na~+/K~+-ATP活性和ATP含量有升高的趋势,SGLT1、GLUT2、AMPK-α1和mTOR mRNA的表达量显著升高(P0.05)。[结论]日粮中添加亮氨酸能够有效提高IUGR仔猪小肠葡萄糖吸收和能量代谢关键酶活性及基因表达量,促进小肠的发育,改善小肠葡萄糖吸收和能量代谢状态。
[Abstract]:[objective] to study the effect of leucine on glucose absorption and energy metabolism in small intestine of weaned piglets with intrauterine growth retardation (IUGR). [methods] NBW (normal birth weight) and 16 newborn piglets (IUGR) were selected from 16 sows in normal delivery and weaned at 14 days of age. They were fed basal diet (containing 1.45% leucine) or leucine diet (containing 1.80% leucine) and were randomly divided into 4 groups. The activity of jejunal enzymes and the mRNA expression of related enzymes were measured. [results] the expression of SGLT1 and PK mRNA in the jejunum of IUGR piglets decreased significantly as well as the activity of MDH in mitochondria and the content of ATP in mitochondria, and the expression of SGLT1 and PK mRNA decreased significantly in the jejunum of IUGR piglets. However, the expression of AMPK- 伪 1 and mTOR mRNA was significantly decreased in IUGR piglets, and the activities of AKP, MDH and SDH in mitochondria of IUGR piglets were significantly increased by leucine supplementation. The activity of Na ~ + -ATP and the content of ATP were significantly increased in SGLT1GLUT2AMPK- 伪 1 and mTOR of IUGR piglets, and the mRNA expression of GLUT2AMPK- 伪 1 and mTOR was significantly increased in IUGR piglets. [conclusion] Leucine supplementation in diet can effectively increase the activity of key enzymes and gene expression of glucose absorption and energy metabolism in the small intestine of IUGR piglets, promote the development of small intestine and improve the state of glucose absorption and energy metabolism in the small intestine of IUGR piglets.
【作者单位】：南京农业大学动物科技学院;
【基金】：国家重点基础研究发展计划项目(2012CB124703)
【分类号】：S828.5

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