miR-126-5p在沙子岭猪睾丸组织差异表达分析及功能预测

发布时间：2018-06-17 06:48

本文选题：沙子岭猪 + miR--p　；参考：《家畜生态学报》2017年07期

【摘要】：利用实时荧光定量PCR(Real-time PCR)检测miR-126-5p在沙子岭猪睾丸组织8个不同发育时期(E90、D1、D30、D60、D90、D120、D150、D180)表达量,以了解其在动物睾丸发育及精子生成中的作用。利用miRanda、TargetScan和mirWalk 3个数据库预测miR-126-5p靶基因并取交集,利用David在线软件分析miR-126-5p靶基因的生物功能。结果表明,miR-126-5p在胚胎期(E90-D1)睾丸组织中高表达,而在生长期(D1-D90)表达量下调,但在沙子岭猪性成熟(D120)后表达量又上调,其中D1表达量最高,D90表达量最低,且二者与其他7个时期均差异极显著(P0.01);D30、E90、D180相互之间差异不显著(P0.05),且分别与其余各时期差异极显著(P0.01);D120与D30差异显著(P0.05),与其余各时期差异极显著(P0.01);D150与D60之间差异不显著(P0.05),二者分别与其余各时期差异极显著(P0.01)。miR-126-5p靶基因参与细胞增殖、凋亡、分化、细胞通讯、迁移及蛋白质转运等生物学过程(P0.001);靶基因分子功能包括蛋白二聚体活性、配体依赖性核受体活性(P0.001);KEGG分析显示miR-126-5p靶基因参与TGF-β、MAPK、细胞黏附等信号通路(P0.01)。结合miR-126-5p在睾丸组织不同发育时期表达差异结果及其靶基因功能预测结果,miR-126-5p可能参与沙子岭猪生殖细胞分化及精子生成调控,本研究为miR-126-5p在动物精子生中的作用及其调控机制的研究提供依据。
[Abstract]:The expression of miR-126-5p in 8 different development stages of testis of Shailing pig was detected by real-time fluorescence quantitative PCR real-time PCRR. The expression of miR-126-5p was studied in order to understand the role of miR-126-5p in testicular development and spermatogenesis. The miR-126-5p target gene was predicted and intersected by three databases of miRandaScan and mirWalk, and the biological function of miR-126-5p target gene was analyzed by David online software. The results showed that the expression of miR-126-5p was highly expressed in the testis of E90-D1 at embryonic stage, but down-regulated in the growth stage of D1-D90. However, the expression level was up-regulated after sexual maturation of SHALING pig (D120). The highest expression of D1 and the lowest of the expression of D90 were observed. There were significant differences between the two groups and the other seven periods. There was no significant difference between the two groups and other periods, and there was no significant difference between P0.01D120 and D30, and there was no significant difference between P0.01D150 and D60. There were significant differences between the two groups and other stages. The target genes of P0.01N. MiR-126-5p were involved in cell proliferation. The biological processes of apoptosis, differentiation, cell communication, migration and protein transport are as follows: the target gene functions include protein dimer activity, ligand dependent nuclear receptor activity P0.001 and KEGG analysis shows that miR-126-5p target gene is involved in TGF- 尾 MAPK and cell adhesion signal pathway P0.01. Combined with the results of differential expression of miR-126-5p at different stages of testicular development and prediction of target gene function, miR-126-5p may be involved in the regulation of germ cell differentiation and spermatogenesis in Shailing pig. This study provides evidence for the study of the role of miR-126-5p in animal spermatogenesis and its regulatory mechanism.
【作者单位】：湖南农业大学动物科学技术学院;畜禽遗传改良湖南省重点实验室;湘潭市畜牧水产局;
【基金】：国家现代农业产业技术体系建设专项资金(CARS-36)
【分类号】：S828

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