马鼻疽补体结合反应检测方法的改进

发布时间：2018-06-17 07:42

本文选题：马鼻疽 + 补体结合试验　；参考：《中国预防兽医学报》2017年07期

【摘要】：为配合国家中长期动物疫病防治规划(2012-2020)对马鼻疽的监测任务,本研究对马鼻疽诊断技术(农业行业标准,NY/T 557-2002)中补体结合试验的体系进行了优化和改进。改进后的方法使用商品化的抗原、补体、溶血素,保证了实验体系的稳定;与原方法相比,该方法将反应的总体系从2.5 m L缩小为0.75 m L,阴阳性血清的稀释度从1∶10降至1∶20,阳性血清、阴性血清、抗原、溶血素、补体、红细胞的使用量均缩减了5倍左右,降低了检测成本,同时将检测用具由试管改为2 m L聚丙烯塑料管,方便了实验室的操作和生物废弃物的无害化处理。利用上述改进的方法和原方法分别对来自河北、北京、天津和上海的47份马血清进行了检测,结果相同,以此验证了两种方法在反应条件和稳定性方面是一致的。
[Abstract]:In order to cooperate with the task of monitoring horse mallex in the national long-term animal disease prevention and control program (2012-2020), this study optimized and improved the complement binding test system in the diagnosis technique of equine mallex (NYR / T557-2002). The improved method uses commercial antigens, complements and hemolysins to ensure the stability of the experimental system. The total reaction was reduced from 2.5 mL to 0.75 mL, and the dilution of yin-yang serum was reduced from 1:10 to 1: 20. The usage of positive serum, negative serum, antigen, hemolysin, complement and erythrocyte were all reduced by about 5 times. The detection cost was reduced, and the test equipment was changed from test tube to 2 mL polypropylene plastic tube, which was convenient for laboratory operation and harmless treatment of biological waste. 47 horse serum samples from Hebei, Beijing, Tianjin and Shanghai were detected by the improved method and the original method, respectively. The results were the same. The results showed that the reaction conditions and stability of the two methods were the same.
【作者单位】：内蒙古农业大学兽医学院;中国农业科学院哈尔滨兽医研究所兽医生物技术重点实验室;新疆维吾尔自治区动物卫生监督所;
【基金】：农业部动物卫生行业标准项目-马鼻疽诊断技术修订(2016-25-1)
【分类号】：S855.12

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