2011～2015年中国Ⅰ类新城疫病原学监测与遗传进化分析

发布时间：2018-06-17 12:24

本文选题：Ⅰ类新城疫病毒 + 主动监测　；参考：《病毒学报》2017年02期

【摘要】：为了研究Ⅰ类新城疫病毒在中国的分布及分子演化特征,2011~2015年按照《国家动物疫病监测与流行病学调查计划》,随机从国内26个省市945个采样点共采集家禽口咽、泄殖腔拭子及环境样本53 176份,通过病毒分离和RT-PCR对中国I类新城疫病毒的分布和分子特征进行了调查分析。结果显示,2011~2015年共分离到I类新城疫病毒1 472株,其中,2011年病毒分离率最高,随后病毒分离率有所下降;Ⅰ类新城疫病毒分布范围广,在中国22个省市均分离到病毒,病毒主要分布于华东、华中、华南和西南地区;活禽批发市场和农贸市场的I类新城疫阳性率明显高于养殖场和屠宰场;分离株主要来源于鸡(1 182株),鸭、鹅、鸽子和环境样本中也存在I类新城疫病毒;所有分离株可划分为3个基因型(1~3型),其中基因3型为近年来中国流行的主要基因型。
[Abstract]:In order to study the distribution and molecular evolution of class I Newcastle disease virus in China, according to the National Animal Disease Surveillance and Epidemiology investigation Plan from 2011 to 2015, a total of 945 samples were collected randomly from 26 provinces and cities in China. A total of 53,176 cloacal swabs and environmental samples were collected. The distribution and molecular characteristics of Newcastle disease virus I in China were investigated by virus isolation and RT-PCR. The results showed that 1 472 strains of class I Newcastle disease virus were isolated from 2011 to 2015, among them, the isolation rate of class I Newcastle disease virus in 2011 was the highest, and the isolation rate of class I Newcastle disease virus decreased, and the virus of class I Newcastle disease virus was widely distributed in 22 provinces and cities of China, and the virus was isolated in 22 provinces and cities of China. The positive rate of class I Newcastle disease in live poultry wholesale markets and farmers' markets was significantly higher than that in farms and slaughterhouses, and the isolates were mainly from 1 182 strains of chicken, ducks and geese. There was also class I Newcastle disease virus in pigeons and environmental samples, and all isolates could be divided into three genotypes, of which gene 3 was the main genotype prevalent in China in recent years.
【作者单位】：中国动物卫生与流行病学中心;
【基金】：公益性行业(农业)科研专项(项目号:201303033),题目:鸡新城疫防控技术研究与示范
【分类号】：S855.3

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