一株鸡源志贺氏菌的分离、鉴定及耐药性分析

发布时间：2018-06-18 08:41

本文选题：志贺氏菌 + 分离　；参考：《中国家禽》2017年04期

【摘要】：为了对鸡源志贺氏菌性腹泻进行快速准确的诊断和耐药性研究,试验对由贵州省动物疫病研究室提供的一株鸡源志贺氏菌进行进一步的分离、PCR鉴定及药敏试验。结果显示,经细菌分离培养、革兰氏染色及生化鉴定试验,该菌符合志贺氏菌的特点及其生化特性;PCR检测结果显示,针对志贺氏菌ipa H基因设计的一对引物可扩增出393 bp的目的条带,与预期大小一致,具有高度的特异性;对其耐药性的研究显示,该株志贺氏菌对参试的20种药物产生不同程度的耐药性。研究鸡源志贺氏菌的分离与鉴定中所结合的PCR方法具有快速、简单、特异性强的特点,能够迅速确定病原菌,并在药敏试验中发现了针对该菌敏感性较高、疗效更好的药物。结果为鸡源志贺氏菌耐药性的有关研究与临床用药提供了科学的参考依据。
[Abstract]:In order to diagnose Shigella gallinaceus diarrhea quickly and accurately and to study its drug resistance, a strain of Shigella from chicken was further isolated and identified by PCR and drug sensitivity test, which was provided by Animal epidemic Disease Laboratory of Guizhou Province. The results showed that after isolation, culture, Gram staining and biochemical identification test, the bacteria accorded with the characteristics of Shigella and its biochemical characteristics. A pair of primers designed for the ipa H gene of Shigella spp can amplify 393 BP of the target band, which is consistent with the expected size and has a high specificity. Shigella spp. Shigella has different drug resistance to 20 drugs tested. The PCR method used in isolation and identification of Shigella spp. From chicken was rapid, simple and specific, and it was able to identify pathogenic bacteria quickly. In the drug sensitivity test, a drug with higher sensitivity and better curative effect was found for Shigella spp. The results provided a scientific reference for the study and clinical use of Shigella chicken-origin drug resistance.
【作者单位】：贵州大学动物科学学院;贵州省动物疫病研究室;贵州省动物疫病预防控制中心;贵州瑞特新科农牧科技有限公司;
【基金】：贵州省科学技术厅农业攻关项目(黔科合NY[2015]3009-1号) 贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队项目(黔科合人才团队[2015]4016号) 贵州省农业委员会科技计划项目[2016-01号]
【分类号】：S852.61

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