转Fat-1基因猪外源基因遗传稳定性分析

发布时间：2018-06-18 11:52

本文选题：Fat-基因 + 转基因猪　；参考：《河南农业科学》2017年11期

【摘要】：测定转Fat-1基因猪外源基因遗传稳定性,为转基因猪育种选择优质新材料提供基础数据。采用PCR和Southern印迹杂交方法对转Fat-1基因传代生产仔猪进行检测,了解Fat-1基因在猪基因组中的整合情况,统计传代猪转基因阳性比率,与自然基因单等位基因正常遗传比率进行差异显著性分析。结果显示,F2代传代仔猪Fat-1基因阳性比率为57.14%,F3代传代仔猪阳性比率为46.67%。F1代公猪外源Fat-1基因是1拷贝基因,传代配种母猪使用非转基因母猪,仔猪阳性比率与自然基因单等位基因正常遗传比率(50%)差异不显著。Fat-1基因在猪育种传代过程中遗传力没有下降,转Fat-1基因公猪能够稳定遗传生产后代猪。
[Abstract]:The genetic stability of exogenous gene in transgenic pig Fat-1 was determined to provide basic data for selection of high quality new materials for transgenic pig breeding. Polymerase chain reaction (PCR) and Southern blotting were used to detect Fat-1 gene integration in porcine genome. The difference between normal genetic ratio of single allele and natural gene was analyzed. The results showed that the positive rate of Fat-1 gene was 57.14 in F _ 2 generation and 46.67% in F _ 3 generation. The exogenous Fat-1 gene of F _ 1 generation boar was 1 copy gene. There was no significant difference between the positive ratio of piglets and the normal genetic ratio of natural gene single allele (50%). The heritability of Fat-1 gene did not decrease during pig breeding and passage, and the transgenic boar with Fat-1 gene could produce offspring steadily.
【作者单位】：湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室;
【基金】：转基因生物新品种培育重大专项(2016ZX08006002-006) 湖北省农业科技创新中心项目(2017-620-004-001) 湖北省农业科学院竞争性项目(2016jzxjh013) 湖北省技术创新专项重大项目(2016ABA117)
【分类号】：S828

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