反刍动物饲料小肠可吸收氨基酸流量简化评定技术的研究

发布时间：2018-06-20 01:55

本文选题：CNCPS + 降解率　；参考：《山东农业大学》2015年硕士论文

【摘要】：本研究以康奈尔净碳水化合物与蛋白质体系(CNCPS,Cornell Net Carbohydrate and Protein System)对RUP部分小肠可吸收氨基酸流量的预测方程为基础。对18份饲料原料测定预测RUP小肠可吸收氨基酸流量所需数据,并以体外法测定各时间点降解残渣的小肠消化率。通过所测数据,以CP的降解率和小肠消化率对CNCPS进行简化,并比较其可靠性。研究表明:(1)精料和粗料均以8h的CP瘤胃降解率与有效降解率相关性最强,二者呈线性相关,精料方程为Y=12.65229+0.82807X,r=0.98993,P0.0001;粗料方程为:Y=10.96738+0.88625X,r=0.97964,P=0.0006;(2)精料2h降解残渣的小肠消化率与有效消化率相关性最强,线性回归方程为:Y=0.02632+0.87927X,r=0.97029,P0.0001;粗料8h降解残渣的小肠消化率与有效消化率相关性最强,线性回归方程为:Y=-0.00224+0.95997X,r=0.99489,P0.0001;(3)提出了3种简化方案,并对3种简化方案的可靠性进行评估,结果表明,精粗料均以8h的降解率和8h降解残渣的小肠消化率对RUP小肠可吸收氨基酸流量预测结果与CNCPS评定结果相关性最强,精料方程为:Y=-0.05564+1.40925X,r=0.99955,P0.0001;粗料方程为:Y=0.00197+2.11988X,r=0.99946,P0.0001。与CNCPS预测结果的均方差分别为0.25和0.005,差异性分析P值分别为0.9995和0.9971,说明简化模型是可行的。其次,本研究对DM、CP、NDF、ADF和OM的瘤胃动态降解率的测定进行简化,结果表明,精料以16h的降解率与有效降解率相关性最强,方程为:Y=0.44750+0.87007X,r=0.97121,P0.0001;粗料以8h的降解率与有效降解相关性最强,方程为:Y=14.23080+0.84717X,r=0.99104,P0.0001。
[Abstract]:Based on the predictive equation of Connell's net carbohydrate and protein system (CNCPS, Cornell Net Carbohydrate and Protein System) on the absorbable amino acid flow in the small intestine of RUP, 18 feed raw materials were used to determine the number of amino acid absorbable amino acid flow in the small intestine and to determine the degradation residue at each time point in vitro. The intestinal digestibility of the small intestine was simplified by the measured data of CP and intestinal digestibility, and the reliability of CNCPS was compared. The results showed that (1) the correlation between the degradation rate of CP rumen and the effective degradation rate of the fine material and coarse material was the strongest, the two had linear correlation, the formula of fine material was Y=12.65229+0.82807X, r=0.98993, P0.0001, and the coarse material equation was Y=10.96738+0.88625X, r=0.97964, P=0.0006; (2) the correlation of small intestinal digestibility with effective digestibility of 2H degradation residue is the strongest, linear regression equation is Y=0.02632+0.87927X, r=0.97029, P0.0001, and the digestion rate of small intestine with 8h degradation residue is the strongest with the effective digestibility, and the linear regression equation is Y=-0.00224+0.95997X, r=0.99489, P. 0.0001 (3) (3) 3 simplified schemes were proposed and the reliability of the 3 simplified schemes was evaluated. The results showed that the prediction results of the RUP absorbable amino acid flow of the small intestine were the strongest with the degradation rate of 8h and the intestinal digestibility of the 8h degradation residue, and the equation of fine material was Y=-0.05564+1.40925X, r=0.99955, P0.0001; The equation of coarse material is: the mean square deviation of Y=0.00197+2.11988X, r=0.99946, P0.0001. and CNCPS is 0.25 and 0.005 respectively. The difference analysis of P values is 0.9995 and 0.9971, respectively, indicating that the simplified model is feasible. Secondly, this study simplifies the determination of the dynamic degradation rate of the rumen in DM, CP, NDF, ADF and OM. The results show that the essence is degraded by 16h. The correlation between the rate and the effective degradation rate is the strongest. The equation is Y=0.44750+0.87007X, r=0.97121, P0.0001, and the degradation rate of 8h with the effective degradation is the strongest. The equation is Y=14.23080+0.84717X, r=0.99104, P0.0001..
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S816

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