不同浓度EPA和DHA对C2C12成肌细胞增殖及凋亡的影响

发布时间：2018-06-20 02:43

本文选题：二十碳五烯酸 + 二十二碳六烯酸　；参考：《中国畜牧杂志》2017年01期

【摘要】：本试验旨在研究二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对体外培养的C2C12成肌细胞增殖和凋亡的影响。用不同终浓度DHA和EPA(0、6.25、12.5、25、50、100μmol/L)分别作用C2C12细胞12、24、48 h后,用CCK-8法检测细胞的增殖情况;用不同终浓度DHA和EPA(0、50、100μmol/L)分别作用C2C12细胞48 h后,用TUNEL法检测细胞凋亡情况。结果表明:终浓度为50、100μmol/L的EPA处理细胞48 h,C2C12细胞增殖能力受到显著抑制,但不同浓度DHA处理组细胞的增殖能力无明显变化。此外,EPA处理C2C12细胞后,TUNEL法显示凋亡细胞数增加,且100μmol/L的EPA处理组细胞凋亡最明显。然而,与对照相比,DHA处理组细胞无明显凋亡。上述结果表明,EPA能抑制C2C12成肌细胞增殖并促进其凋亡,而DHA对细胞增殖和凋亡无明显影响。
[Abstract]:The aim of this study was to investigate the effects of eicosapentaenoic acid (EPA) and 22 carbohexaenoic acid (DHA) on the proliferation and apoptosis of C2C12 myoblasts in vitro. C2C12 cells were treated with different final concentrations of DHA and EPA-06.2512.5 and 2550mol / L respectively for 48 h, then the proliferation of C2C12 cells was detected by CCK-8 method, and C2C12 cells were treated with DHA and EPA-050100 渭 mol / L for 48 h, and the apoptosis of C2C12 cells was detected by Tunel method. The results showed that the proliferation of C2C12 cells treated with final concentration of 50100 渭 mol / L was significantly inhibited, but the proliferation ability of cells treated with different concentrations of DHA was not significantly changed. In addition, the number of apoptotic cells was increased by Tunel method after treated with EPA, and the apoptosis was most obvious in 100 渭 mol / L EPA treatment group. However, there was no significant apoptosis in the DHA treated group compared with the control group. These results suggest that EPA can inhibit the proliferation and promote the apoptosis of C2C12 myoblast, but DHA has no effect on the proliferation and apoptosis of C2C12 myoblast.
【作者单位】：武汉轻工大学动物营养与饲料科学湖北省重点实验室;中南民族大学生命科学学院;
【基金】：湖北省自然科学资助项目(2015CFB514) 湖北省高等学校优秀中青年科技创新团队计划项目(T201508)
【分类号】：S816

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