Ⅰ型菌毛对F18ac大肠杆菌粘附性能的影响

发布时间：2018-06-22 11:34

本文选题：Fac大肠杆菌 + Ⅰ型菌毛　；参考：《中国预防兽医学报》2017年02期

【摘要】：为研究Ⅰ型菌毛在F18ac大肠杆菌(F18ac E.coli)粘附过程中的作用,本研究利用λ-Red同源重组系统构建了F18ac E.coliⅠ型菌毛主亚基编码基因fim A缺失突变株(F18ac△fim A),以野生株为对照,通过体外易感细胞粘附试验和生物被膜(BF)形成定量试验,探索Ⅰ型菌毛在F18ac E.coli粘附宿主细胞过程中的作用。体外易感细胞粘附结果显示,经8%甘露糖封闭Ⅰ型菌毛受体或fim A基因缺失后,F18ac E.coli对易感仔猪上皮细胞IPEC-1的粘附能力均显著下降。BF结晶紫定量试验显示,F18ac△fim A菌株形成BF的能力显著下降,而F18ac△fim A/pfim A回补株的粘附能力以及生物被膜形成能力均得到了恢复。本研究表明Ⅰ型菌毛是F18ac E.coli重要的粘附素,介导了F18ac E.coli对仔猪易感细胞的粘附过程。本研究为进一步阐释F18ac E.coli致病机制中多毒力因子的作用提供了实验依据。
[Abstract]:In order to study the role of fimbriae type I in the adhesion process of Escherichia coli F18ac (F18ac E.coli), the mutant F18ac fim A), (F18ac fim A),) was constructed using 位 -Red homologous recombination system. By means of susceptibility cell adhesion test and biofilm formation quantitative test in vitro, the role of type I fimbriae in the adhesion of F18ac E.coli to host cells was explored. The results of susceptible cell adhesion in vitro showed that, The adhesion ability of F18ac E.coli to IPEC-1 of susceptible piglet epithelial cells was significantly decreased after 8% mannose blocking of type I fimbria receptor or fim A gene deletion. The results of quantitative analysis of BF crystal violet showed that the ability of F18ac fim A strain to form BF was significantly decreased. The adhesion ability and biofilm formation ability of F18ac fim A/pfim A compensator were recovered. This study showed that type I pili is an important adhesion hormone of F18ac E.coli, which mediates the adhesion process of F18ac E.coli to susceptible cells of piglets. This study provides an experimental basis for further elucidating the role of polyvirulence factors in the pathogenesis of F18ac E.coli.
【作者单位】：渭南职业技术学院;扬州大学兽医学院;
【基金】：陕西省科技新星项目(2015KJXX-97) 渭南市科技创新项目(2013KYJ-3)
【分类号】：S852.61

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