沙门菌烈性噬菌体的分离鉴定及生物学特性的试验

发布时间：2018-06-22 15:19

本文选题：沙门菌 + 噬菌体　；参考：《中国兽医杂志》2017年06期

【摘要】：从商品肉鸭场采集鸭粪及垫料,以实验室保存的沙门菌为宿主菌,采用双层平板法分离到1株烈性噬菌体。对分离到的噬菌体进行纯化、效价测定、裂解谱测定、形态观察,并且进行了最佳感染复数、温度跟p H值稳定性和一步生长曲线的生物学特性研究。结果表明,该噬菌体形成的蚀斑均匀透亮,效价能达到109PFU/m L以上,并能裂解多株沙门菌菌株,为宽裂解谱噬菌体,透射电镜显示,该噬菌体为长尾噬菌体;最佳感染复数为0.001;一步生长曲线测定表明,该噬菌体的潜伏期为15 min,暴发期为30 min,暴发量约为320;该噬菌体的温度、p H值耐受性良好;紫外线照射60 min后效价降低3个梯度,由此可见该噬菌体分离株可作为潜在开发的生物消毒剂菌株。
[Abstract]:1 strains of phage were isolated with Salmonella from the commercial meat duck farm, and 1 strains of phage were isolated by the laboratory preserved Salmonella. The isolated phage was purified, titer, cleavage spectrum and morphological observation, and the optimum number of infection, temperature and P H value stability and one step growth curve were produced. The results showed that the plaque formed by the phage was uniform and bright, the potency could reach more than 109PFU/m L, and the multiple strains of Salmonella could be cracked. The phage was a wide lysate phage. The transmission electron microscope showed that the phage was a long tail phage, the optimum number of infection was 0.001, and the one step growth curve showed that the latent period of the phage was 1. 5 min, the outbreak period was 30 min, the outbreak amount was about 320; the temperature of the phage, P H was well tolerated, and the titer decreased by 3 gradient after ultraviolet radiation 60 min, thus the bacteriophage isolate could be used as a potential biodisinfectant strain.
【作者单位】：青岛农业大学动物科技学院;
【基金】：山东省现代农业产业技术体系—牛产业创新团队项目(SDAIT-09-03) 山东省高等学校优势学科创新团队培养计划项目
【分类号】：S852.61

【相似文献】