KAP16基因对细毛羊重要经济性状的遗传效应分析

发布时间：2018-06-24 20:49

本文选题：KAP基因 + SNPs　；参考：《西南农业学报》2017年02期

【摘要】：为了分析KAP16基因的遗传多态性,获取相关毛性状的DNA标记,为细毛羊在分子育种上提供依据,采用重测序技术和PCR-SSCP技术,验证KAP16候选基因的2个SNPs在中国美利奴(新疆型)群体中的遗传多态性。同时,运用SAS 8.1软件进行最小二乘方差分析方法,研究其与毛性状的关联性。结果表明,C41006938T突变点得到3种基因型为AA、AB和BB型,该群体中C41006938T突变点基因型频率为0.22、0.47和0.31;A和B等位基因频率为0.46和0.54,其中B等位基因为优势等位基因。T41007938C基因位点AA和AB基因型频率为0.77和0.23;A和B等位基因频率分别为0.89和0.11,A基因为优势等位基因。χ2检验表明,C41006938T和T41007938C位点均处于哈代温伯格平衡状态(P0.05)。C41006938T和T41007938C突变位点的多态信息含量分别为0.37和0.18,所以中国美利奴(新疆型)群体在该基因的遗传变异处在中等水平。C41006938T不同基因型对体格大小和鉴定时体重有显著影响(P0.05);T41007938C不同基因型对纤维直径纤维直径变异系数有极显著影响(P0.01),对剪毛量和弯曲有显著影响(P0.05);C41006938T和T41007938C组合基因型对剪毛量有极显著影响(P0.01),对纤维直径纤维直径变异系数有显著影响(P0.05)。
[Abstract]:In order to analyze the genetic polymorphism of KAP16 gene and obtain the DNA markers of relevant wool traits, and to provide the basis for molecular breeding of fine wool sheep, the techniques of re-sequencing and PCR-SSCP were used to analyze the genetic polymorphisms of KAP16 gene. To verify the genetic polymorphism of two SNPs of KAP16 candidate gene in Chinese Merino (Xinjiang type) population. At the same time, the correlation between SAS 8.1 and wool traits was studied by using least square analysis of variance (LS-ANOVA). The results showed that the three genotypes of C41006938T were AAAAB and BB. The genotype frequencies of C41006938T were 0.22, 0.47 and 0.31 A and B, respectively, and the frequencies of genotype A and B were 0.77 and 0.23A and B, respectively. The frequencies of allele B were dominant allele. T41007938C genotype AA and AB were 0.77 and 0.23A, respectively. 蠂 2 test showed that C41006938T and T41007938C were in Hardy Weinberg equilibrium (P0.05) .C41006938T and T41007938C were 0.37 and 0.18, respectively. The genetic variation of the gene was at medium level. C41006938T genotypes had significant effects on body size and body weight in identification (P0.05) different genotypes of T41007938C had extremely significant effects on the coefficient of variation of fiber diameter (P0.01), and had significant effects on the amount of hair shearing and bending. Significant effects (P0.05) C41006938T and T41007938C combination genotype had a significant impact on the shearing amount (P0.01), and on the fiber diameter coefficient of variation (P0.05).
【作者单位】：新疆农业大学动物科学学院;新疆畜牧科学院畜牧研究所分子遗传重点实验室;
【基金】：国家自然科学基金(31360543,31460593) 农业部体系项目(CARS-40) 新疆农业大学产学研联合培养研究生示范基地项目(xjaucxy-yjs-20152011)
【分类号】：S826

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