硒对六价铬鸡中毒肝损伤的影响研究

发布时间：2018-06-24 21:52

  本文选题：铬(Ⅵ) + 硒　； 参考：《山东农业大学》2017年硕士论文

【摘要】：近年来,对机体微量元素的研究已经成为热点。本实验的目的是探究不同浓度的硒对六价铬造成的肝损伤的促进或者抑制作用。首先进行急性动物实验,测得K_2Cr_2O_7得LD50为369mg/kg,选择6%LD50作为本次试验的攻毒剂量,即为22.14mg/kg。慢性动物实验,选用105只7日龄海兰褐鸡,随机分为七组,通过饮水供毒,第Ⅰ组为空白组,饮用去离子水,第Ⅱ组饮水中添加22.14mg/kg的K_2Cr_2O_7,第Ⅲ组添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(0.31 mg/kg),第Ⅳ组添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(0.63 mg/kg),第Ⅴ组添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(1.25 mg/kg),第Ⅵ组添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(2.50 mg/kg),第Ⅶ组添加K_2Cr_2O_7(22.14mg/kg)+Na_2SeO_3(5.00mg/kg),各组试剂连续添加42d。在验的14d,28d,42d采集肝脏样品,检测肝体比、丙二醛(MDA)含量、谷胱甘肽(GSH)含量、总超氧化物歧化酶(T-SOD)活性、Ca~(2+)-ATP酶活性和线粒体膜电位(MMP)变化,观察肝组织病理切片。结果表明,饮水中添加六价铬会导致鸡肝脏生长发育受阻,肝组织切片能观察到病理损伤。六价铬引起MDA含量增加,GSH含量减少,T-SOD活性和Ca~(2+)-ATP活性降低,MMP下降,并且各组数据与对照组差异极显著(P0.01)。同时,饮水中添加六价铬的同时分别补充0.31mg/kg、0.62mg/kg和1.25mg/kg硒时,能够抑制六价铬所引起的肝脏损伤,降低MDA含量,增加GSH含量,升高T-SOD和Ca~(2+)-ATP酶活性,使MMP上升,其中以添加0.63 mg/kg的硒效果最佳。饮水中添加六价铬的同时补充2.50 mg/kg和5.00mg/kg的硒时,能加重六价铬所诱导的肝脏损伤,肝脏组织中MDA含量进一步增多,GSH含量降低,T-SOD和Ca~(2+)-ATP酶活性降低,MMP下降。实验结果表明,一定剂量的K_2Cr_2O_7能导致肝损伤,而且饮水中添加适量的硒对Cr(Ⅵ)造成的肝损伤具有保护作用,过量的硒则加重Cr(Ⅵ)造成的肝损伤。
[Abstract]:In recent years, the research on trace elements has become a hot spot. The aim of this study was to investigate the effects of selenium at different concentrations on liver injury induced by hexavalent chromium. First, the acute animal experiment was carried out, and the LD50 was determined to be 369 mg / kg, and 6LD50 was selected as the dose of 22.14 mg / kg. In the chronic animal experiment, 105 7-day-old Hailan brown chickens were randomly divided into seven groups, which were fed by drinking water. The first group was a blank group, drinking deionized water. 绗�鈪＄粍楗�姘翠腑娣诲姞22.14mg/kg鐨凨_2Cr_2O_7,绗�鈪㈢粍娣诲姞K_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(0.31 mg/kg),绗�鈪ｇ粍娣诲姞K_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(0.63 mg/kg),绗�鈪ょ粍娣诲姞K_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(1.25 mg/kg),绗�鈪ョ粍娣诲姞K_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(2.50 mg/kg),绗�鈪︾粍娣诲姞K_2Cr_2O_7(22.14mg/kg) Na_2SeO_3(5.00mg/kg),鍚勭粍璇曞墏杩炵画娣诲姞42d. Liver samples were collected on the 14th day after 28 days. The liver body ratio, malondialdehyde (MDA) content, glutathione (GSH) content, total superoxide dismutase (T-SOD) activity and mitochondrial membrane potential (MMP) were observed. The results showed that adding hexavalent chromium to drinking water could lead to the inhibition of liver growth and development, and pathological damage could be observed in liver tissue sections. The content of MDA and GSH were increased by hexavalent chromium. The activity of T-SOD and Ca ~ (2) -ATP decreased, and the data of each group were significantly different from the control group (P0.01). At the same time, adding hexavalent chromium and adding 0.31 mg / kg 0.62 mg / kg and 1.25mg/kg selenium in drinking water could inhibit the liver injury induced by hexavalent chromium, decrease the content of MDA, increase the content of GSH, increase the activities of T-SOD and Ca ~ (2) -ATPase, and increase the activity of MMP. Among them, the effect of adding 0.63 mg/kg selenium was the best. Addition of chromium hexavalent to drinking water supplemented with selenium of 2.50 mg/kg and 5.00mg/kg increased the liver damage induced by Cr, and further increased the content of GSH in the liver and decreased the activities of T-SOD and Ca ~ (2) -ATPase. The experimental results showed that a certain dose of K _ S _ 2Cr _ 2O _ 7 could lead to liver injury, and the addition of proper amount of selenium to drinking water could protect the liver from Cr (鈪，

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