鸭RIG-1启动子克

发布时间：2018-06-29 11:52

本文选题：RIG- + 启动子　；参考：《浙江大学学报(农业与生命科学版)》2017年01期

【摘要】：RIG-1属于细胞内蛋白,与细胞增殖、分化和天然抗免疫功能密切相关。为研究RIG-1在鸭胚胎期免疫器官发育中的作用,本研究克隆得到鸭RIG-1启动子并做生物信息学分析,应用实时荧光定量PCR技术检测RIG-1基因以及预测得到的多个转录因子在鸭胚胎免疫器官发育过程中的表达模式。结果表明,扩增得到鸭RIG-1基因启动子4 372bp。序列分析表明,该基因启动子存在典型的TATA-box、CAAT-box调控元件,有IRF-1、RXR、RAR、AP1、NF-κB、SP1、IL6及Pax-2等多个转录因子结合位点。此外,RIG-1启动子预测发现了一个CpG岛,GC含量65.8%。定量结果发现,RIG-1在鸭胚胎免疫器官中表达呈动态性,具有不同的表达模式;并在法氏囊中的表达量高于脾脏和胸腺。聚类热图显示,只有在法氏囊中RIG-1与IRF-1、RXR、AP1、NF-κB、IL6的mRNA表达量具有相似的表达模式,这可能是由于在鸭胚胎期法氏囊具有较为完整的结构与功能,暗示它们可能为RIG-1的转录因子;而在法氏囊、脾脏和胸腺中,RIG-1与IRF-1、NF-κB基因都有较为相似的表达模式,在3个组织当中均被聚类在一起,说明IRF-1和NF-κB可能参与调控RIG-1的表达。研究结果为探索鸭RIG-1基因转录调控、表达,与在细胞增殖、分化、天然抗病毒免疫方面的功能提供了依据和方向。
[Abstract]:RIG-1 belongs to intracellular protein and is closely related to cell proliferation, differentiation and natural anti-immune function. In order to study the role of RIG-1 in the development of duck immune organs at embryonic stage, duck RIG-1 promoter was cloned and analyzed by bioinformatics. Real-time fluorescent quantitative PCR was used to detect the expression patterns of RIG-1 gene and several transcription factors in the development of duck embryonic immune organs. The results showed that the promoter of duck RIG-1 gene was 4372 BP. Sequence analysis showed that the promoter had a typical TATA-box CAAT-box regulatory element and several transcription factor binding sites, such as IRF-1, RXR, RARX, AP1, NF- 魏 B, SP1, Pax-2, and so on. In addition, a CpG insular GC content of 65.8% was found in the prediction of RIG-1 promoter. The quantitative results showed that the expression of RIG-1 was dynamic in the immune organs of duck embryos and had different expression patterns, and the expression level in bursa of Fabricius was higher than that in spleen and thymus. Clustering thermogram showed that only in bursa of Fabricius did RIG-1 and IRF-1RXRHAP1NF-kappa IL-6 have similar expression patterns, which may be due to the relatively complete structure and function of bursa of Fabricius at embryonic stage, suggesting that RIG-1 may be a transcription factor of RIG-1. In the bursa of Fabricius, spleen and thymus, the expression pattern of RIG-1 and IRF-1NF-1NF- 魏 B gene was similar, and it was clustered in three tissues, indicating that IRF-1 and NF- 魏 B might be involved in regulating the expression of RIG-1. The results provide the basis and direction for exploring the transcription regulation, expression and function of duck RIG-1 gene in cell proliferation, differentiation and natural antiviral immunity.
【作者单位】：四川农业大学动物遗传育种研究所;
【基金】：国家自然科学基金(31301964) 四川省科技支撑应用基础项目(2015JY0110)
【分类号】：S834

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