宁海黄鸡和广西黄鸡KLF7基因多态性及其与屠体性状的相关分析

发布时间：2018-06-29 21:26

本文选题：单核苷酸多态性 + Kruppel样因子基因(KLF)　；参考：《农业生物技术学报》2017年02期

【摘要】：Kruppel样因子7(Kruppel-like factor,KLF7)是参与脂肪代谢的一个因子,其对鸡(Gallus gallus)生长和屠体性状的相关研究较少。本研究采用600K Affymetrix Axiom Chicken SNP芯片技术,研究鸡KLF7基因单核苷酸多态性(SNPs)及其与屠体性状的相关性。本实验经对宁海黄鸡和广西黄鸡各59个个体进行基因分型,在KLF7基因上共发现38个SNPs位点,其中4个SNPs位点位于外显子上。除宁海黄鸡T16936C、A18000C、C33569T、T34758C和T40474G位点以及广西黄鸡A18715C和T45125A位点外,其余位点均处于Hardy-Weinberg平衡状态,多态信息含量显示,宁海黄鸡和广西黄鸡KLF7基因大多数位点属中度多态。各SNPs位点与宁海黄鸡和广西黄鸡屠体性状相关性分析结果表明,分别有7个和6个SNPs位点对部分屠体性状有显著或极显著影响(P0.05或P0.01)。其中,A18000C和T45125A两个SNPs位点对宁海黄鸡和广西黄鸡屠体性状均有显著或极显著影响(P0.05或P0.01)且宁海黄鸡有AA、AB和BB 3种基因型,而广西黄鸡仅有AB和BB两种基因型。宁海黄鸡A18000C位点AB型的胸肌率极显著高于BB型(P0.01),T45125A位点AB型的半净膛率和全净膛率极显著高于AA型(P0.01),显著高于BB型(P0.05),AB型的腹脂重和腹脂率显著高于AA型和BB型(P0.05);广西黄鸡A18000C位点AB型的胸肌率显著高于BB型(P0.05),T45125A位点AB型的屠宰率、半净膛率、全净膛重和全净膛率显著高于BB型(P0.05)。研究结果表明,KLF7基因对鸡屠体性状有一定的基因效应,为鸡屠体性状具有潜力的候选基因,可作为鸡屠体性状的分子标记辅助选择。
[Abstract]:Kruppel-like factor 7 (KLF7) is one of the factors involved in fat metabolism. There are few studies on the relationship between growth and carcass traits in chicken (Gallus gallus). The single nucleotide polymorphism (SNPs) of chicken KLF7 gene and its association with carcass traits were studied by 600K Affymetrix Axiom Chicken SNP chip technique. In this study, 38 SNPs loci were found on the KLF7 gene by genotyping of 59 individuals in Ninghai Yellow Chicken and Guangxi Yellow Chicken respectively, and 4 SNPs loci were found on exon. With the exception of T16936CA18000 C33569THN T34758C and T40474G, and Guangxi Yellow Chicken A18715C and T45125A, all the other loci were in Hardy-Weinberg equilibrium. The polymorphic information content showed that the majority of KLF7 loci in Ninghai chicken and Guangxi yellow chicken were moderately polymorphic. The results of correlation analysis between SNPs loci and carcass traits of Ninghai Yellow Chicken and Guangxi Yellow Chicken showed that 7 SNPs loci and 6 SNPs loci had significant or extremely significant effects on some carcass traits (P0.05 or P0.01). The two SNPs loci of A18000C and T45125A had significant or extremely significant effects on carcass traits of Ninghai Yellow Chicken and Guangxi Yellow Chicken (P0.05 or P0.01), and Ninghai Yellow Chicken had three genotypes of AAAAB and BB, while Guangxi Yellow Chicken had only AB and BB genotypes. The breast muscle rate of A18000C genotype AB in Ninghai yellow chicken was significantly higher than that of BB type (P0.01) and AB type (P0.01), and the percentage of total purge was significantly higher than that of AA type (P0.01), and was significantly higher than that of BB type (P0.05), abdominal fat weight and abdominal fat rate of BB type AB (P0.05), while that of BB type (P0.01) was significantly higher than that of AA type and BB type (P0.05). The breast muscle rate of A18000C genotype AB was significantly higher than that of BB type (P0.05), and the slaughter rate of AB type at T45125A locus was significantly higher than that of BB type (P0.05). The rate of half-bore, full-bore weight and total-purged bore was significantly higher than that of BB-type (P 0. 05). The results showed that KLF7 gene had a certain gene effect on chicken carcass traits, which was a candidate gene with potential for chicken carcass traits and could be used as a molecular marker assisted selection for chicken carcass traits.
【作者单位】：浙江大学动物科学学院;
【基金】：浙江省重大科技专项重大农业项目(No.2012C12906-15)
【分类号】：S831.2

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