番茄红素对山羊精液冷冻保存效果的影响

发布时间：2018-06-29 21:41

本文选题：番茄红素 + 山羊　；参考：《西北农业学报》2017年03期

【摘要】：为研究番茄红素对山羊精液冷冻保存效果的影响,在山羊精液冷冻稀释液中添加不同质量浓度(0、0.5、1.0、2.0、4.0mg/mL)的番茄红素,检测冷冻-解冻后精子的活率、顶体完整率、质膜完整率、线粒体活性,并用试剂盒测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活性。结果表明,1.0mg/mL组的冷冻保存效果最好,精子活率、顶体完整率、质膜完整率和线粒体活性分别达到52.04%、51.33%、53.57%和49.31%,显著高于对照组,且SOD、CAT、GSH-Px的活性也显著高于对照组;0.5mg/mL组与2.0mg/mL组的精子活率、顶体完整率、质膜完整率、线粒体活性及CAT和GSH-Px活性差异不显著;4.0mg/mL组的精子活率、顶体完整率、质膜完整率、线粒体活性及SOD和CAT活性与对照组差异不显著。因此,在山羊精液冷冻稀释液中加入1.0mg/mL的番茄红素对山羊精液具有显著的保护作用,能进一步提高山羊冻精品质。
[Abstract]:In order to study the effect of lycopene on the cryopreservation of goat semen, lycopene of different concentration (0 0. 5% 1. 0% 1. 0 ~ 2. 0 mg / mL) was added to the frozen diluent of goat semen. The sperm motility, acrosome integrity rate and plasma membrane integrity rate were measured after freezing and thawing. The activities of superoxide dismutase (SOD), catalase (cat) and glutathione peroxidase (GSH-Px) were measured with the kit. The results showed that the cryopreservation effect of 1.0 mg / mL group was the best, the sperm motility, acrosome integrity rate, plasma membrane integrity rate and mitochondrial activity were 52.0443% and 49.31%, respectively, which were significantly higher than those in the control group, and the activity of GSH-Px in the SOD CATN GSH-Px group was significantly higher than that in the 0.5 mg / mL group and the 2.0 mg / mL group in the control group. There was no significant difference in sperm motility, acrosomal integrity, plasma membrane integrity, mitochondrial activity, SOD and cat activity between groups of 4.0 mg / mL and 4.0 mg / mL. Therefore, the addition of 1.0 mg / mL lycopene into the frozen diluent of goat semen has a significant protective effect on goat semen, and can further improve the quality of goat frozen semen.
【作者单位】：西北农林科技大学动物科技学院;拉萨市动物疫病预防控制中心;榆林学院陕西省陕北绒山羊工程技术研究中心;
【基金】：国家绒毛用羊产业技术体系(CARS-40-13) 陕西省科技统筹创新工程计划(2014KTDZ02-01,2015KTTSNY04-03)~~
【分类号】：S827

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