巴马小型猪对高致病性猪繁殖与呼吸综合征病毒的敏感性评价

发布时间：2018-06-30 20:58

  本文选题：巴马小型猪 + 高致病性猪繁殖与呼吸综合征病毒　； 参考：《动物医学进展》2017年01期

【摘要】：为评价巴马小型猪对高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)的敏感性,本研究选择PRRSV抗体阴性的16头巴马小型猪和17头本地二元杂交猪作为研究对象,分别分为低剂量接毒组、高剂量接毒组、对照组1和对照组2,低剂量、高剂量接毒组肌肉注射接种PRRSV NVDC-JXA1强毒株,对照组1和接毒组混合饲养,对照组2分开饲养作为空白对照。接毒后每天测量体温,观察精神、食欲、死亡等情况,死亡动物剖检病变,攻毒后每隔7d采血用RT-PCR法检测病原,连续观察21d。结果绝大部分猪体温升高到41℃以上,且有3个以上温次,所有接种及混养动物都死亡,巴马小型猪死亡时间在8d~17d,二元杂交猪在7d~13d,死亡动物出现肺充血、出血、实变,扁桃体、淋巴结、肝脏、肾脏、皮下有出血等症状,病原RTPCR检测为阳性,说明攻毒动物死于HP-PRRS,表明巴马小型猪对PRRSV NVDC-JXA1强毒株非常敏感,可用于PRRS活疫苗的检验。
[Abstract]:In order to evaluate the sensitivity of Bama miniature pigs to highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSv), 16 Bama minipigs with PRRSV negative antibody and 17 local binary crossbred pigs were selected as the study subjects. High dose group, control group 1 and control group 2, low dose, high dose group were injected with PRRSv NVDC-JXA1 virulent strain intramuscularly, control group 1 was mixed with inoculated group, control group 2 was fed as blank control. Body temperature, mental state, appetite, death and so on were taken every day. Pathological changes of dead animals were examined. Blood samples were collected every 7 days to detect the pathogen by RT-PCR and observed continuously for 21 days. Results the body temperature of most pigs rose above 41 鈩，

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