表达牛乳铁蛋白肽的重组乳酸乳球菌对雏鸡抗鸡白痢沙门氏菌感染的效果研究

发布时间：2018-07-01 11:31

本文选题：牛乳铁蛋白肽 + 重组乳酸乳球菌　；参考：《中国预防兽医学报》2017年03期

【摘要】：为探索表达牛乳铁蛋白肽的重组乳酸乳球菌pAMJ399-LFBA/MG1363对雏鸡抗鸡白痢沙门氏菌感染效果的影响,本实验将该重组乳酸乳球菌饲喂1日龄雏鸡,连续饲喂14 d,并设立空白对照组,第7 d时经灌服鸡白痢沙门氏菌同时感染实验组和对照组,连续观察7 d;分别于攻菌前以及攻菌后的不同天数采集血清及盲肠黏液,检测结果显示攻菌后饲喂重组乳酸乳球菌组雏鸡血清中IgG以及黏液中SIgA的含量极显著高于空白对照组。对各组雏鸡的心脏、肝脏、脾脏及十二指肠进行病理组织学检查,结果显示饲喂重组乳酸乳球菌组各器官的病变情况相对较轻,而且雏鸡盲肠扁桃体中非特异免疫信号通路中相关分子TLR4、My D88、NF-κB、TNF-α、IL-8、IL-6等的m RNA表达相对较高。以上结果表明,表达牛乳铁蛋白肽的重组乳酸乳球菌在抗鸡白痢沙门氏菌感染的过程中可以起到保护雏鸡免受感染的作用。
[Abstract]:In order to explore the effect of recombinant Lactococcus lactis pAMJ399-LFBA-MG1363 expressing lactoferrin peptide on the infection of Salmonella pullorum in chicks, the recombinant Lactococcus lactis was fed to 1-day-old chicks for 14 days, and a blank control group was set up. On the 7th day, Salmonella pullorum was administered to both experimental group and control group, and the serum and cecum mucus were collected before and after inoculation for 7 days. The results showed that the levels of IgG in serum and Siga in mucus of chicks fed with recombinant Lactococcus lactis after inoculation were significantly higher than those in control group. The pathological examination of heart, liver, spleen and duodenum of the chicks in each group showed that the pathological changes of organs in the group fed with recombinant Lactococcus lactis were relatively mild. Moreover, the mRNA expression of TLR4My D88My D88Cecal tonsil non-specific immune signaling pathway in chicks was relatively high, such as NF- 魏 B, TNF- 伪, TNF- 伪, IL-8, IL-6, and so on. The results indicated that the recombinant Lactococcus lactis expressing lactoferrin peptide could protect chicks from infection in the process of resisting Salmonella pullorum infection.
【作者单位】：东北农业大学;
【基金】：国家自然科学基金项目(31672461) 哈尔滨市科技创新人才基金(2014RFXXJ084)
【分类号】：S858.31

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