CTGF和FGF-2在不同年龄段牦牛肺脏的分布研究

发布时间：2018-07-03 18:07

  本文选题：牦牛 + 肺脏　； 参考：《甘肃农业大学》2017年硕士论文

【摘要】：实验目的:本研究旨在探讨牦牛肺脏对高原低氧环境的应答机制。为了研究牦牛肺脏结构及其对低氧适应性的关系,本研究选择对肺脏结缔组织和平滑肌增生具有特定作用的CTGF(Connective Tissue Growth Factor)和FGF-2(Fibroblast Growth Factors-2)两种因子。本研究初步探讨CTGF与FGF-2对牦牛肺脏低氧适应性结构形成中的作用。实验方法:采用EVG特殊染色方法观察初生、3-5月龄和成年牦牛肺脏内纤维成分的分布情况;免疫组化技术对CTGF和FGF-2在三个年龄段牦牛肺脏内准确的分布时间、位置和表达量进行研究。结果:⑴初生、3-5月龄和成年牦牛肺脏中,胶原纤维主要分布在支气管各级分支和肺动脉周围。弹性纤维分布于肺动脉外膜与中膜的交界处。三个年龄段相比,3-5月龄组支气管各级和肺动脉的肌层增厚明显。⑵在牦牛肺脏支气管和细支气管中,CTGF主要表达在上皮细胞,其中3-5月龄组均表达最强(P0.05),初生次之,成年最弱,但初生和成年无显著差异性(P0.05)。CTGF少量分布在肺血管内皮。在肺小动脉(直径为50um)中,三个年龄组CTGF表达呈缓慢降低趋势(P0.05)。CTGF在3-5月龄组肺动脉(100um~200um)表达最强(P0.05),成年次之,初生最少(P0.05)。3-5月龄组肺动脉(200um)CTGF表达强度最强,成年次之,初生最少(P0.05)。⑶在支气管各级分支中,FGF-2主要分布在上皮细胞和平滑肌细胞。3-5月龄组支气管表达程度最强(P0.05),初生次之(P0.05),成年最弱(P0.05)。3-5月龄组细支气管表达最强(P0.05),初生次之且与成年组无显著性差异(P0.05)。在各级肺动脉中,FGF-2在3-5月龄组肺小动脉(50um)的表达量最高,初生次之,成年组极低(P0.05)。初生、3-5月龄以及成年三组肺动脉(100um~200um)的FGF-2表达量缓慢增加(P0.05)。3-5月龄组肺动脉(200um)表达量最高(P0.05),初生最少(P0.05)。结论:⑴初生、3-5月龄和成年牦牛肺脏的支气管、细支气管及其分支,小动脉(50um)、肺动脉(100um~200um)以及大动脉(200um)管壁均有大量胶原纤维和弹性纤维分布,且血管壁内平滑肌较厚,造成肺动脉及其分支的血管壁均较厚,血管强度高。⑵CTGF主要分布于各年龄段牦牛肺内支气管及其分支的上皮细胞、肺动脉内皮细胞,且在3-5月龄分布最多,说明CTGF可能参与了牦牛气道和肺动脉的形成。⑶FGF-2主要分布于各年龄段牦牛肺内支气管及其分支的上皮细胞、肺动脉内皮细胞;在肺内支气管外膜平滑肌和肺动脉平滑肌中也有大量分布;在3-5月龄的分布量最多。说明该因子参与了牦牛气道和肺动脉的结构的形成。
[Abstract]:Objective: to investigate the mechanism of yak lung response to high altitude hypoxia. In order to study the relationship between lung structure and hypoxia adaptation of yak, two factors, CTGF (Connective tissue growth Factor) and FGF-2 (fibroblast growth Factors-2), which play a specific role in pulmonary connective tissue and smooth muscle hyperplasia, were selected in this study. The purpose of this study was to investigate the role of CTGF and FGF-2 in the formation of hypoxic adaptive structure in yak lung. Methods: using EVG special staining method to observe the distribution of fiber components in the lungs of the newborn and adult yaks, and to determine the exact distribution time of CTGF and FGF-2 in the lungs of the three age groups. Position and expression were studied. Results the collagen fibers were mainly distributed in the bronchi branches and around the pulmonary artery in the lung of the yak at the age of 3-5 months and adult yak. Elastic fibers are distributed at the junction of the adventitia and the medial membrane of the pulmonary artery. The myometrium thickening of bronchi and pulmonary artery in the three age groups was significantly higher than that in the 3-5 month group. CTGF was mainly expressed in the epithelial cells in the bronchi and bronchioles of the yak. The expression of CTGF was the highest in the 3-5 month group (P0.05), followed by the primary age group, and the weakest in adult group. However, there was no significant difference between primary and adult (P0.05). CTGF was distributed in pulmonary vascular endothelium. In pulmonary arterioles (diameter: 50um), the expression of CTGF in three age groups showed a slow decreasing trend (P0.05). The expression of CTGF was the strongest in the pulmonary artery (100um~200um) of 3-5 months old group (P0.05), followed by the adult group (P0.05), the lowest (P0.05) .3-5 month old group (200um) expression intensity was the highest, and the adult group was the second. FGF-2 mainly distributed in epithelial cells and smooth muscle cells. 3-5 months old group (P0.05), newborn group (P0.05), adult weakest (P0.05) .3-5 months old group bronchiole expression (P0.05), primary stage of birth. There was no significant difference between the two groups (P0.05). The expression of FGF-2 was the highest in the pulmonary arterioles (50um) of 3-5 months old group, followed by the newborn group, and was very low in the adult group (P0.05). The expression of FGF-2 in pulmonary artery (100um~200um) of 3 to 5 months old and adult group increased slowly (P0.05). The expression of 200um was the highest in the group of 3-5 months of age (P0.05), and the lowest in newborn group (P0.05). Conclusion the bronchioles, bronchioles and their branches, arterioles (50um), pulmonary arteries (100um~200um) and great arteries (200um) in the lung of the yak between 3-5 months and 3 to 5 months of age have a large number of collagen fibers and elastic fibers, and the smooth muscle of the vascular wall is thicker. The wall of pulmonary artery and its branches were thicker. The high intensity of CTGF was mainly distributed in the epithelial cells of the bronchi and its branches of the lung of yak in all ages, and the endothelial cells of the pulmonary artery were most distributed at the age of 3 to 5 months. These results suggest that CTGF may be involved in the formation of airway and pulmonary arteries. 3FGF-2 mainly distributes in epithelial cells and pulmonary artery endothelial cells in the lung bronchi and its branches of yak in all ages. There were also a large number of distributions in pulmonary adventitia smooth muscle and pulmonary artery smooth muscle, and the distribution was the most in 3-5 months old. It is suggested that this factor is involved in the formation of airway and pulmonary arteries in yak.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823.85
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本文编号：2094547