松辽黑猪四种基因多态性与背膘厚的关联分析

发布时间：2018-07-03 16:48

本文选题：松辽黑猪 + MC4R　；参考：《吉林农业大学》2017年硕士论文

【摘要】：背膘厚是家猪重要的经济性状,同时也是地方猪种选育过程中主要的育种指标。为深入研究松辽黑猪群体背膘厚的遗传基础,以173头松辽黑猪背最长肌组织、四点背膘处的脂肪组织为试验样本,对MC4R、HDAC1、LEP及OLR1进行基因多态性检测,进而分析其与背膘厚、肌内脂肪含量间的关联性,以期为降低背膘厚、提高瘦肉率提供理论基础及分子标记。本研究结果如下:饲养差异:放牧组的胸腰结合处背膘厚、腰荐结合处背膘厚及平均背膘厚显著低于舍饲组。性状分析:肌内脂肪含量与肩部最厚处背膘厚间存在强相关,与平均背膘厚间存在显著相关;体重、肩部最厚、六七肋、胸腰结合、腰荐结合处背膘厚及平均背膘厚间均存在强相关。多态性:MC4R、HDAC1、LEP及OLR1四个候选基因在松辽黑猪群体中均发现多态位点,且均处于Hardy-Weinberg平衡状态,遗传多态性均为中度多态,遗传变异程度均处于中等水平。经分析发现,HDAC1基因10806位点处GA多态性与六七肋、腰荐结合处、最厚肋处的背膘厚密切相关。OLR1基因9011位点处TC、9012位点处CA均与肌内脂肪间存在关联。9070位点处GA多态性与四点背膘厚间存在显著联系。MC4R基因、LEP基因在松辽黑猪群体中与各点背膘厚、肌内脂肪含量间未显示存在任何相关性。表达量:HDAC1基因高背膘厚组在六七肋、胸腰结合处脂肪组织中的mRNA表达量显著高于低背膘厚组(P0.05),OLR1基因高背膘厚组在四点背膘组织中的mRNA表达量均高于低背膘厚组且差异显著(P0.05)。MC4R、LEP基因两组在四点处脂肪组织中的mRNA表达量间均无显著性差异。
[Abstract]:Backfat thickness is an important economic trait in domestic pigs, and it is also the main breeding index in the breeding process of local pigs. In order to study the genetic basis of backfat thickness in Songliao black pig population, the genetic polymorphisms of MC4RN HDAC1LEP and OLR1 were detected by using the longissimus dorsi muscle tissue of 173 Songliao black pigs and adipose tissue at four-point backfat, and the genetic polymorphisms of MC4RHDAC1LEP and OLR1 were analyzed. The correlation of intramuscular fat content in order to provide theoretical basis and molecular marker for reducing backfat thickness and increasing lean meat percentage. The results were as follows: the feeding difference: the backfat thickness of thoracolumbar joint, backfat thickness and average backfat thickness of the grazing group were significantly lower than those of the house-fed group. Character analysis: there was strong correlation between intramuscular fat content and backfat thickness at the thickest part of shoulder, and significant correlation between intramuscular fat content and average backfat thickness. There was strong correlation between backfat thickness and average backfat thickness. Polymorphic loci were found in four candidate genes of HDAC1LEP and OLR1 in Songliao Black Pig population, and they were all in Hardy-Weinberg equilibrium. The genetic polymorphisms were moderately polymorphic, and the degree of genetic variation was at the medium level. The genetic polymorphism at 10806 locus of HDAC1 gene was found to be associated with the combination of six or seven ribs and waist recommendation. The backfat thickness of the thickest rib was closely related to the backfat thickness of the most thick rib. There was a significant association between the 9011 locus of OLR1 gene and the TCQ9012 locus CA. There was a significant association between the polymorphism of GA at the locus of .9070 and the backfat thickness of the four-point backfat. There was a significant association between the backfat thickness and the backfat thickness at each point in the Songliao Black Pig population. There was no correlation between intramuscular fat content. HDAC1 gene was expressed in HDAC1 group with high backfat thickness. The mRNA expression level in thoracolumbar binding adipose tissue was significantly higher than that in low backfat thickness group (P0.05). The mRNA expression of OLR1 gene in high backfat thickness group was higher than that in low backfat thickness group and the difference was significant (P0.05). MC4RNLEP gene expression level was significantly higher in four-point fat group than in low-backfat thickness group (P0.05). There was no significant difference in mRNA expression between tissues.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S828

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