荔波瑶山鸡TGFβ3基因多态性与繁殖性状的相关性

发布时间：2018-07-04 07:40

本文选题：TGFβ基因 + 基因多态位点　；参考：《西南农业学报》2017年08期

【摘要】：【目的】为探明荔波瑶山鸡TGFβ3基因与繁殖性状的关系,寻找可作为鸡繁殖性状的遗传标记。【方法】针对TGFβ3基因设计6对引物,采用PCR测序法筛选多态位点,并利用荔波瑶山鸡母系60个个体进行繁殖性状的关联分析。【结果】荔波瑶山鸡TGFβ3基因有17个多态位点,TGFβ3基因53(T→C)、1653(C→T)和1755(A→G)位点各300日龄产蛋量、首次就巢日龄、首次就巢持续时间、首次就巢至重新开产间隔和300日龄就巢次数间呈显著或极显著相关(P0.05或P0.01);3343(C→T)与首次就巢持续时间、首次就巢至重新开产间隔和300日龄就巢次数均呈显著或极显著相关(P0.05或P0.01);3540(C→T)与开产日龄、300日龄产蛋量和首次就巢持续时间均呈显著或极显著相关(P0.05或P0.01);4786(C→T)和7471(G→A)分别与300日龄产蛋量和首次就巢日龄均呈显著相关(P0.05);7263(C→T)与首次就巢持续时间呈显著相关(P0.05)。【结论】TGFβ3基因53(T→C)、1653(C→T)、1755(A→G)、3343(C→T)、3540(C→T)、4786(C→T)、7263(C→T)和7471(G→A)与就巢性状关联显著,可作为降低就巢性提高繁殖力的辅助选择标记。
[Abstract]:[objective] to explore the relationship between TGF 尾 3 gene and reproductive traits in Libo Yao chicken, and to search for genetic markers for reproductive traits. [methods] six pairs of primers were designed for TGF 尾 3 gene, and PCR sequencing was used to screen polymorphic loci. [results] there were 17 polymorphic loci of TGF- 尾 3 gene (TGF- 尾 3) 53 (T) 1653 (T) 1653 (C T) and 1755 (A G) for 300 days of egg production in Libo Yao Shan Chicken. For the first time, there was a significant or extremely significant correlation between the interval from first nest to reopening and the number of nests at 300 days of age (P0.05 or P0.01). There was a significant or extremely significant correlation between the number of nests (P0.05 or P0.01) 3540 (C / T) and the number of eggs laid at 300 days (P0.05 or P0.01) and the duration of the first nest at 300 days (P0.05 or P0.01), respectively (P0.05 or P0.01), and (P0.05 or P0.01) significant or extremely significant correlation between the number of nests and the number of nests at 300 days of age (P0.05 or P0.01), as well as the number of eggs laid at 300 days of age (P0.05 or P0.01). (P 0.05) 7263 (C T) and the first nest duration (P 0.05). [conclusion] the TGF- 尾 3 gene 53 (T C) 1653 (C TGP T) 1755 (A G) 3343 (C T) 3540 (C Tu T) 7263 (C T) and 7471 (G) 7263 (T) and 7471 (G), respectively, were significantly correlated with the egg production at 300 days of age and the first nest age (P0.05). [conclusion] TGF- 尾 3 gene 53 (T) 1653 (C / T) 1755 (A / G) 3343 (C / T) 3540 (C / T) (C / T) 7263 (C / T) and 7471 (G / A), respectively. The correlation of nest traits was significant. It can be used as an auxiliary selection marker to reduce the fecundity and improve fecundity.
【作者单位】：贵州省畜牧兽医研究所;
【基金】：贵州省联合基金项目“瑶山鸡ONECUT1基因的CDS区克隆;差异表达及与生产性状关联分析”[黔科合LH字(2015)7067] 贵州省基金项目“瑶山鸡就巢性选育关键基因筛选”[黔科合J字(2014)2102] 贵州省农业动植物育种项目“瑶山鸡新品系选育”[黔农育专字(2014)008] 贵州省农业科学院2014年博士基金项目“瑶山鸡STAT5基因变异检测及与产蛋性状关联分析”
【分类号】：S831

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