多糖-硒化多糖复方抗氧化活性的比较

发布时间：2018-07-04 21:42

本文选题：多糖-硒化多糖复方 + 自由基清除能力　；参考：《畜牧兽医学报》2017年02期

【摘要】：为了研制抗氧化中药多糖制剂,在前期研究的基础上,选择抗氧化活性较强的枸杞多糖(LBP)、白术多糖(AMP)、当归多糖(CAP)、党参多糖(CPP)、大蒜多糖(GP)、百合多糖(LP)、五味子多糖(SCP)、硒化枸杞多糖(sLBP)、硒化白术多糖(sAMP)、硒化当归多糖(sCAP)为组分药,以糖含量按照多糖-硒化多糖9∶1的比例组成18个复方,比较它们的抗氧化活性。体外试验,比较18个复方对3种自由基的清除能力;体内试验,雏鸡免疫新城疫苗时分别注射高、低剂量的3个复方,测定血清3种抗氧化酶和MDA含量的动态变化。体外试验结果显示,18个复方的5个浓度对DPPH自由基、羟自由基和ABTS自由基均有不同程度的清除能力,LBP-sAMP、CAP-sLBP和CAP-sAMP的能力较强。体内试验结果显示,6个复方组在4个时间点的血清CAT、SOD、GSH-Px活性多高于或显著高于对照组,MDA含量均低于(多显著低于)对照组;CAP-sLBP组的3种抗氧化酶活性均为最高、MDA含量最低。18个复方均有不同程度的抗氧化活性,CAP-sLBP在18个复方中的抗氧化活性最强,可以考虑作为多糖类抗氧化制剂的候选方。
[Abstract]:In order to develop antioxidant Chinese medicine polysaccharide preparation, on the basis of previous studies, Lycium barbarum polysaccharides (LBP), Atractylodes macrocephala polysaccharides (AMP), Angelica sinensis polysaccharides (CAP), Codonopsis polysaccharide (CPP), garlic polysaccharides (GP), lily polysaccharides (LP), Schisandra chinensis polysaccharides (SCP), Selenated Lycium barbarum polysaccharides (sLBP), Selenated Rhizoma Atractylodes polysaccharides (sAMP) and Angelica selenidum polysaccharides (SCAP) were selected. Eighteen compounds were composed by sugar content according to the ratio of polysaccharides to polysaccharides selenide 9:1, and their antioxidant activities were compared. In vitro experiment, the scavenging ability of 18 compounds to three kinds of free radicals was compared, and in vivo experiment, three compounds of high and low doses were injected to chicks respectively to determine the dynamic changes of serum antioxidant enzymes and MDA contents. The results of in vitro experiments showed that the 5 concentrations of 18 compounds had different scavenging ability to DPPH radical, hydroxyl radical and Abts radical. LBP-sAMP-CAP-sLBP and CAP-sAMP were stronger than those of LBP-sAMP-CAP-sLBP and CAP-sAMP. The results of in vivo test showed that the activities of GSH-Px in serum of six compound groups were higher than or significantly higher than those of the control group at 4 time points. The activities of three antioxidant enzymes in CAP-sLBP group were all the highest and the content of MDA was higher than that of the control group (mostly significantly lower than that of the control group). The antioxidation activity of CAP-sLBP was the highest in 18 compounds. It may be considered as a candidate for polysaccharides antioxidants.
【作者单位】：南京农业大学中兽医学研究室;
【基金】：国家自然科学基金(31272596) 公益性行业(农业)科研专项(201403051)
【分类号】：S853.7

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