从江香猪不同组织脂蛋白脂酶mRNA的表达及其酶活性研究

发布时间：2018-07-05 04:12

本文选题：从江香猪 + 酶活性　；参考：《畜牧与兽医》2017年03期

【摘要】：为进一步探究脂蛋白脂酶(LPL)基因的功能,揭示其对从江香猪肉质性状的影响,本研究采用荧光定量RT-PCR技术结合酶活性检测方法分析了从江香猪不同组织LPL基因表达水平及活性规律。结果显示,LPL基因在从江香猪大脑、心脏、肝脏、脾脏、肺、肾脏、大肠、背肌、子宫和卵巢10种组织内均有表达;其中,背肌和心脏组织中表达量较高,子宫和肺组织中表达量较低,且背肌中LPL mRNA表达量显著高于其他各组织(P0.05)。从江香猪4种组织LPL活性分析结果表明,背肌组织中的LPL活性显著高于子宫和心脏组织(P0.05),说明背肌中脂肪沉积能力高于子宫和心脏组织,研究结果将为今后分析该基因对从江香猪肉质性能的影响奠定基础。
[Abstract]:In order to further explore the function of lipoprotein lipase (LPL) gene and reveal its effect on meat quality of Congjiang Xiang pig, In this study, the expression level and activity of LPL gene in different tissues of Congjiang Xiang pig were analyzed by fluorescence quantitative RT-PCR and enzyme activity assay. The results showed that LPL gene was expressed in 10 tissues including brain, heart, liver, spleen, lung, kidney, large intestine, dorsal muscle, uterus and ovary. The expression of LPL mRNA in the dorsal muscle was significantly higher than that in the other tissues (P0.05). The results of LPL activity analysis showed that the LPL activity in dorsal muscle was significantly higher than that in uterus and heart (P0.05), indicating that the ability of fat deposition in dorsal muscle was higher than that in uterus and heart. The results will lay a foundation for the analysis of the effect of the gene on the meat quality of Congjiang Xiang pig.
【作者单位】：贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室贵州大学动物科学学院贵州省动物遗传育种与繁殖重点实验室;贵州省生猪健康养殖工程技术研究中心;贵州省天柱县动物疫病预防控制中心;
【基金】：贵州省工程中心建设项目(黔科合农C字[2011]4022号) 贵州大学引进人才科研项目(贵大人基合字[2013]21号)
【分类号】：S828

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