一株尿素酶阴性产气巴氏杆菌的分离鉴定及生物学特性分析

发布时间：2018-07-05 13:08

本文选题：产气巴氏杆菌 + 尿素酶阴性　；参考：《中国预防兽医学报》2017年03期

【摘要】：本研究从猪流产胎儿中分离到一株形似产气巴氏杆菌菌株并命名为ZY14013,对其进行生化鉴定、16S rDNA序列分析和药物敏感性分析。生化鉴定结果表明,ZY14013除尿素酶阴性外,其它生化特征与产气巴氏杆菌模式菌株ATCC 27883以及其它分离株具有相似生化特征。遗传进化分析表明ZY14013与ATCC 2788以及16株现存分离株16S rDNA核苷酸同源性分别为99.2%和99.6%~99.9%,在进化树中形成同一个群。药敏试验表明,分离株ZY14013对青霉素、氟苯尼考和头孢类抗生素敏感,对链霉素、卡那霉素、庆大霉素、氯霉素和氧氟沙星耐药。本研究首次证实了猪产气巴氏杆菌感染在中国大陆的存在,同时,本研究在世界首次分离到尿素酶阴性产气巴氏杆菌,丰富了产气巴氏杆菌的生化特征,为研究尿素酶在产气巴氏杆菌致病性中的作用提供了自然突变体。
[Abstract]:In this study, a strain of Pasteurella aeruginosa was isolated from aborted pig fetus and named ZY14013. The strain was identified by biochemical analysis of 16s rDNA sequence and drug sensitivity analysis. The biochemical characteristics of ZY14013 were similar to those of Pasteurella aerogenes model strain ATCC 27883 and other isolates except urease negative. Genetic evolution analysis showed that the nucleotide homology of 16s rDNA between ZY14013 and ATCC 2788 and 16 existing isolates was 99.2% and 99.6%, respectively. The drug sensitivity test showed that ZY14013 was sensitive to penicillin, florfenicol and cephalosporins, and was resistant to streptomycin, kanamycin, gentamicin, chloramphenicol and ofloxacin. This study first confirmed the presence of Pasteurella aeruginosa infection in the mainland of China. At the same time, we isolated the urease negative Pasteurella aeruginosa for the first time in the world, which enriched the biochemical characteristics of Pasteurella aeruginosa. It provides natural mutants for studying the role of urease in pathogenicity of Pasteurella aerogenes.
【作者单位】：云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室;
【基金】：云南省现代农业生猪产业技术体系建设项目(云财教[2013]160号)
【分类号】：S852.61

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