金华猪和长白猪粪便微生物移植小鼠的肠道古菌结构分析

发布时间：2018-07-08 17:23

本文选题：古菌 + 金华猪　；参考：《动物营养学报》2017年06期

【摘要】：本试验旨在比较肥胖型的金华猪和瘦肉型的长白猪肠道古菌结构的差异,分析菌群移植小鼠肠道古菌结构。采集金华猪和长白猪的新鲜粪便,通过灌胃移植到经广谱抗生素处理过的小鼠肠道中,提取2种猪粪便及其移植小鼠的盲肠内容物总基因组DNA,使用古菌通用引物对古菌16S rRNA的V4区进行PCR扩增,通过Illumina Hiseq测序平台对扩增产物进行高通量测序,使用QIIME等软件对测序序列进行分析统计。结果表明:金华猪和长白猪粪便及其移植小鼠盲肠内容物中的古菌主要包含3个门,分别为奇古菌门(Thaumarchaeota)、广古菌门(Euryarchaeota)和泉古菌门(Crenarchaeota),其中金华猪粪便中广古菌门的丰度高于长白猪;在属水平上主要包含4个已知属,优势属为餐古菌属(Cenarchaeum)。二者移植小鼠的盲肠内容物古菌结构,在门水平上,移植小鼠的奇古菌门比例占70%以上;奇古菌门的古菌几乎全部来源于餐古菌属;在门和属的水平上,金华猪和长白猪粪便移植小鼠的盲肠古菌结构无显著差异(P0.05),但基于操作分类单元(OTU)水平的主成分分析显示出很好的组内聚集。由此可见,奇古菌门的餐古菌属是金华猪和长白猪粪便和其移植小鼠盲肠中的优势古菌;金华猪粪便中广古菌门的丰度高于长白猪粪便,且二者移植小鼠盲肠内容物之间古菌结构在OTU水平上存在一定的差异。
[Abstract]:The purpose of this experiment was to compare the structure of intestinal ancient bacteria between the obese Jinhua pig and the lean Landrace pig, and to analyze the structure of the intestinal ancient bacteria in mice transplanted with the microflora. Fresh feces from Jinhua pig and Landrace pig were collected and transplanted into the intestine of mice treated with broad-spectrum antibiotics. The total genomic DNA of cecum contents of two kinds of pig feces and their transplanted mice were extracted. The V4 region of 16s rRNA was amplified by using the universal primer of archaea, and the high throughput sequencing was carried out by Illumina Hiseq sequencing platform. QIIME and other software were used to analyze the sequence. The results showed that the ancient bacteria in the feces of Jinhua pig and Landrace pig and the caecum contents of transplanted mice mainly contained three phylum, Thaumarchaeota, Euryarchaeota and Crenarchaeota, respectively. The abundance of Phaeophyta in Jinhua pig's feces was higher than that in Landrace. At the generic level, there are mainly 4 known genera, and the dominant genus is Cenarchaeum. The structure of the cecum contents of the two transplanted mice was more than 70% at the gate level, and almost all of the ancient bacteria of the phylum came from the genus Cyanophora; at the level of phylum and genus, the percentage of the bacteria in the gravida was more than 70%, and at the level of phylum and genus, There was no significant difference in the structure of Cecum archaea between Jinhua pig and Landrace pig faecal transplantation mice (P0.05), but the principal component analysis based on the operating classification unit (OTU) level showed a good intra-group aggregation. It can be seen from this that the genus Phaeophora is the dominant ancient bacteria in the feces of Jinhua pig and Landrace pig and the cecum of the transplanted mice, and the abundance of the phylum Ganguopsis in the feces of Jinhua pig is higher than that of the feces of Landrace pigs. There were some differences in the structure of Archaea between the two transplanted mice caecum contents at the level of OTU.
【作者单位】：浙江农业科学院农产品质量标准研究所;北京食品营养与人类健康高精尖创新中心;中国农业大学动物科技学院;
【基金】：浙江省自然科学基金重点项目(LZ15C170001) 北京食品营养与人类健康高精尖创新中心开放基金国家自然科学基金(31402083)
【分类号】：S828

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